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Inhibition effects of thirteen kinds of Chinese herbal medicine on the Malassezia. restricta separated and purified from dandruff in vitro

WANG Jia,WANG Yan-ping,SHI Jian-bo,ZHOU Li-na(School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China)  
Objective To measure the MIC of 13 kinds of Chinese herbal medicine to Malassezia restricta separated and purified from dandruff in vitro.Methods The dandruff was collected from healthy individuals and patients with seborrheic dermatitis.The microscope and physiological and biochemical identification experiment were used to isolate and identify Malassezia.The inhibition effect of 13 kinds of Chinese herb in different concentrations on the growth of M.restricta in vitro was measured through the microdilution method from the relevant standard formulated by NCCLS(National Committee for Clinical Laboratory Standards).Results Thirty-five strains of Malassezia were obtained,which were 3 strains of M.gobosa,3 strains of M.pachydermatis,2 strains of M.slooffiae,2 strains of M.obtusa and 25 strains of M.restricta.MIC for M.restricta:Cortex Dictamni(5 000.0-10 000.0 mg·L-1),Rhizome Alpiniae Officinarum(≥10 000.0 mg·L-1),Rhizome Coptidis(312.5-1 250.0 mg·L-1),Fructus Schisandrae Chinensis(2 500.0-10 000.0 mg·L-1),Cortex Cinnamomi(2 500.0-10 000.0 mg·L-1),Fructus Mume(1 250.0-10 000.0 mg·L-1),Radix et Rhizoma Rhei(625.0-5 000.0 mg·L-1),Herba Houttuyniae(312.5-2 500.0 mg·L-1),Ginger Pinellia(1 250.0-10 000.0 mg·L-1),Dandelion(312.5-5 000.0 mg·L-1),Baishen(5 000.010 000.0 mg·L-1),Violet(2 500.010 000.0 mg·L-1),Folium Mori(5 000.010 000.0 mg·L-1).Conclusions M.restricta is strongly inhibited by Rhizome Coptidis,Fructus Mume,Radix et Rhizoma Rhei,Herba Houttuyniae,Ginger Pinellia and Dandelion.A very important reference for clinical medicine using is provided by this result.
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