Research on the inhibitory effect of quercetin on the growth and proliferation in human colon carcinoma cell line SW480 in vitro
LIN Zeng-hai, MA Tao, MENG Yong. Department of Hepatobiliary Surgery, the First Affiliated Hospital of Shantou University Medical College, Shantou 515041, China
Objective To observe the inhibitory effect on the growth and proliferation of human colon carcinoma SW480 cell line in vitro induced by quercetin. Methods SW480 cells were incubated in vitro by cell biology technique. By means of CCK-8, the effect of quercetin on SW480 cell was studied, the morphological change of SW480 cell was investigated by light microscope and electron microscope. Flow cytometry using propidium iodide (PI) staining was used to determine the cell cycle and cell apoptotic rate. Results Quercetin inhibited the growth of SW480 cell by a time- and dose- dependent manner (P 0.05). The amount of SW480 cell treated by quercetin decreased. Apoptotic corpuscles were found by electron microscope. The apoptosis rate of SW480 cell was improved, and induced cell cycle arrest in G2/M in does-dependent manner of quercetin (P 0.05). Conclusion Quercetin can inhibit the growth of SW480 cell by a time- and dose- dependent manner and induce the apoptosis of SW480 cell in vitro.