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《Chinese Journal of Nephrology, Dialysis & Transplantation》 2004-01
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Genescan analysis of clonality of TCR Vβ repertoire T cell in patients with maintenance hemodialysis

YU Lixin1, MA JUNjie1, YANG Lijian2, LI YaNgqiu2, LIU Qifa3, XU Bing3 1Dept. of Kidney Transplantation, Nan Fang Hospital, Guangzhou, 510515; 2Dept. of Hematology, Jinan University, Guangzhou, 510632; 3Dept. of Hematology, Nan Fang Hospital, Guangzhou, 510515  
Objective: To investigate the clonality of TCR Vb subfamily T cell in the patients with maintenance hemodialysis. Methodology: The CDR3 of TCR Vb 24 subfamily genes were amplified in samples of peripheral blood mononuclear cells before hemodialysis, which were drawn from 11 patients, There were 4 cases of IgA nephropathy (IgAN), 3 of membranous glomerulopathy (MGN) , 3 of membranopoliferative glomerulonephritis (MPGN), and 1 of focal segmental glomerulosclerosis (FSGS), undergoing maintenance hemodialysis. In order to obverse the usage of TCR Vb repertoire, the RT-PCR products were further labeled with fluorescent and analyzed by genescan technique for CDR3 size, to evaluate the clonal expansion and distribution of the detectable TCR Vb subfamily T cells. Results: Only 1-10 TCR Vb subfamily T cells could be identified in all of the patients, and there was only one TCR Vb subfamily (Vb3) T cells detected in 2 cases, respectively expressed as oligoclonality and biclonality. The most frequent expression of TCR Vb genes was Vb 3 (in 8 patients), the second included Vb8, Vb19, Vb22 (in 6 patients), and the third included Vb10, Vb23 (in 5 patients). There was significant negative relationship between the hemodialysis time and expression of TCR Vb subfamily in all patients (r=-0.646,P=0.044) by analysis of linear correlation. Similar results were found respectively in MGN, MPGN and IgAN (F=10.193,P=0.019), but no significant expression of TCR Vb in different primary diseases (F=1.772,P=0.249) by the analysis of variance. Conclusion: The significantly skew distribution and clonal proliferation of TCR Vb subfamily T cells could be found in all of the patients with maintenance hemodialysis, which may indicate that these patients had been stimulated by similar immune stimulus and low cell immune functions. The longer the time of hemodialysis, the less expression of TCR Vb indicated that hemodialysis could be one of the causes of dysfunction of cell immunity in patients with chronic renal failure.
【CateGory Index】: R459.5
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