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《LISHIZHEN MEDICINE AND Materia MEDICA RESEARCH》 2005-09
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Determination and Comparison of the Contents of Total Ginsenoside and Ginsenoside Rb_1 in Twelve Kinds of Panax quinquefolium. L

CHEN Jun-hui, XIE Ming-yong~*, ZHANG Zhi-ming, FU Zhi-hong, NIE Shao-ping(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, China)  
Objective:To develop a quick, convenient, modern analysis method for the determination of ginsenoside Rb_1 in Panax quinquefolium L., to determine the contents of total ginsenoside and ginsenoside Rb_1 of twelve samples and to compare the changes of the determined contents of samples origining from different regions.Methods:The contents of total ginsenoside were determined by UV-spectrophotometry, and the contents of ginsenoside Rb_1 were determined by HPLC. Results:The calibration curve was linear over the concentration range of 62~123 0 μg·ml~(-1) for ginsenoside Rb_1, the correlation coefficient was 0.999. The average recovery of the determination of ginsenoside Rb_1 was 99.2% with the RSD of 1.69%. The contents of total ginsenoside of ten samples are more than 4%, while contents of ginsenoside Rb_1 of ten samples are more than 1%.Conclusion:The developed method was found to be simple, fast, accurate and reliable for the determination of ginsenoside Rb_1.
【CateGory Index】: R284
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