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《LiShiZhen Medicine and Materia Medica Research》 2006-10
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Determination of Ginsenosides in Panax quinquefolium L.and Red Panax quinquefolium L.by RP-HPLC-ELSD

ZHAO Yan~1,LIU Jin-ping~1, LU Dan~1, LI Ping-ya~(1*), MA Xing-long~2(1.Institute of Frontier Medical Science of Jilin University,Jilin,Changchun 130021,China;2.Jilin Provincial Tongbao Chinese Medicinal Materia Scientific & Technological Development Co.Ltd,Jilin,Jingyu 135206,China)  
ObjectiveTo establish a simple method for determination of pseudoginsenosides and ginsenosides in Panax quinquefolium L.and Red Panax quinquefolium L.at the same time.MethodsReverse phase high-performance liquid chromatography(RP-HPLC) method with an evaporative light-scattering detector(ELSD) was performed on a 5μm Diamonsil~(TM) C_(18) column(4.6 mm×250 mm)at ambient temperature.The mobile phase composition was methanol-water(71∶29).The flow rate was at 0.950ml·min~(-1).ELSD parameters were set as follows: nitrogen carrier gas pressure 2.8 Bar,drift tube temperature 40℃.ResultsThe method fulfills all the standard requirements of precision,accuracy and linearity.The recoveries of components at three concentration levels were: P-F_(11) 98.9%,P-RT_5 101.4%,Re 100.5%,Rg_2 100.4%,Rh_1 99.8%,Rg_3 100.0%,Rh_2 100.2% and Rd 103.8%.ConclusionRP-HPLC-ELSD method can give more accurate and sensitive results for the analyses of ginsenosides and pseudoginsenosides,especially for P-F_(11) and P-RT_5 without chromophore,in Panax quinquefolium L.and Red Panax quinquefolium L.
【CateGory Index】: R284.2
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【References】
Chinese Journal Full-text Database 4 Hits
1 LIU Wei-can ZHANG Mei-ping LI Chao-sheng(Jilin Agricultural University,Changchun 130118,China)SUN Chun-yu JIANG Shi-cui LI Chuang WANG Yi(Academy of Traditional Chinese Medicine and Materia Medica of Jilin Province,Changchun 130021 China);Determination of Ginsenosides for diferent parts in Panax quinquefolium L. by HPLC[J];;2010-01
2 PAN Guo-qing1, PING Hua2, FENG Jia3, REN Gui-xing2 (1.Institute of Special Economic Animal and l Plant Sciences, Chinese Academy of Agricultural Sciences, Zuojia 132109; 2.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081; 3.Ginseng Association, Changchun 130021);Determination of individual ginsenoside and malonyl-ginsenoside in different commercial ginseng products[J];Food Science and Technology;2009-01
3 MENG Qiong1,2,QIAN Zheng-ming2,CHEN Zhi-yu1,LI Shao-ping2(1.Chengde Medical University,Chengde 067000,China;2.Institute of Chinese Medical Sciences,University of Macau,Macao SAR,China);HPLC characteristics of Radix Panacis Quinquefolii[J];Chinese Journal of Pharmaceutical Analysis;2010-05
4 YANG Lin,MIAO Yu,YIN Hui-jun,SHI Da-zhuo,CHEN Ke-ji(Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China);Identification of ginsenosides from Panax quinquefolius in Xinyue Capsula by LC-ESI-MS~n[J];Chinese Traditional and Herbal Drugs;2010-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HU Wei1,BAI Shi2,ZHU Tao1,ZHANG Lian-xue21. College of Life Science,Jilin Agricultural University,Changchun 130118,China; 2. College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Study and Optimization of a Method for High-Quality RNA Extraction From Panax ginseng Root Tissues[J];Journal of Jilin Agricultural University;2010-05
2 ZHAO Ying1,YU Fu-lai1,ZHANG Gui-ying2,WANG Bao-gui21.College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China; 2.College of Public Health,Jilin University,Changchun 130021,China;Study on the Anticancer Activities of the Ginsenoside and Clematoside Mixture in vivo[J];Journal of Jilin Agricultural University;2010-06
3 DING BO1,WANG De-lu2*,YIN Jian-qiang1,MA Wei1,ZHOU Guang-yue3,LONG Wen-neng3(1.General Station of Forestry Science and Technology Popularization of Guizhou Province,Guiyang 550001,China;2.Forestry College,Gui-zhou University,Gui-yang 550025,China;3.Forestry Bureau of Duyun City,Guizhou,558000,China);Effects of manganese on chemical qualities in Kuding tea[J];Journal of Jiangsu Forestry Science & Technology;2009-05
4 HUgang, ZHANGyan and XUxiaojun Institute for Geriatric Research, Ministry for Public Health, Beijing 100730, China. Department of Immunology, Peking University Health Science Center, Beijing 100083, China;Anti-oxidant effect of Vigconic Ⅵ-28 capsule, a formulated traditional Chinese medicine, in BALB/c mice[J];Chinese Journal of Geriatric Care;2005-02
5 HE Jia-liang, QIN Cui-li, ZHANG Min(Department of Food & Bioengineering, Henan University of Science and Technology, Luoyang 471003, China);Development of refreshing functional food[J];Journal of Henan University of Science and Technology(Agricultrual Science);2004-02
6 WU Yaoheng1,NING Yizhen2,XU Jianbang3,TAN Yuhui2,WU Yingya2,YAN Ding'an1,LV Guoquan1(1.The Second Clinical College,Guangzhou University of TCM,Guangzhou 510120 Guangdong,China;2.College of Basic Medicine,Guangzhou University of TCM,Guangzhou 510405 Guangdong,China;3.The Second Affiliated Hospital,Guangzhou Medical College,Guangzhou 510260 Guangdong,China);A Study on Shenfu Injection and Lappaconitine Hydrobromide Injection Inducing HL-60 Differentiation and Apoptosis[J];Journal of Guangzhou University of Traditional Chinese Medicine;2008-02
7 CHEN Jun-hui,XIE Ming-yong,WANG Hui-qin,PENG Ri-huang,WANG Yuan-xing(Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047,China);Determination of Ginsenosides in Panax quinquefolium. L by HPLC and Study on Their Fingerprints Analysis[J];Food Science;2005-11
8 YANG He GAO Yu-gang LI Fan-ying ZHANG Lian-xue(Jilin Agriculture University College of Chinese Traditional Medicine,Changchun 130118,China);Research Progress on Biosynthetic Pathway of Terpenoids Containing Ginsenoside and the HMGR[J];China Biotechnology;2008-10
9 ZHOU Jing~1,DU Xiao-Wei~(2*),MA Hong-Yue~3,WU Jun-Kai~2(1.Pharmaceutical College,Nanjing University of Traditional Chinese Medicine,Nanjing 210046,China;(2.Pharmaceutical College,)Heilongjiang University of Chinese Medicine,Harbin 150040,China;3.Chinese Pharmacy College,China Pharmaceutical University,Nanjing 210038,China);Studies on the HPLC Fingerprints of Panax quinquefolium L.[J];LiShiZhen Medicine and Materia Medica Research;2006-12
10 Li Xiaoyong, Zhao Xinfeng, Lu Hongbo, Wang Zhen, Zheng Xiaohui (Department of Medical Chemistry, School of Science of Xi'an Jiaotong University, Xi'an 710061, China);Study on the LC-MS~ (n) method of 24(R)-pseudo-ginenoside in panax quinquefolium L.[J];Journal of Xi'an Jiaotong University(Medical Sciences);2004-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GUI Shuang-ying,ZHOU Ya-qiu (Anhui College of Traditional Chinese Medicine,Anhui Hefei 230038,China);Determination of Ginsenosides in Panax Ginseng by Colorimetric Method[J];Journal of Anhui Traditional Chinese Medical College;2003-04
2 HUANG Hui- qiang* , GUAN Zhong- zhen, HE You- jiang, JIANG Wen- qi, SUN Xiao- fei, LIU Dong- geng, XU Rui- hua, ZHANG Li, ZHOU Zhong- mei, LIN Tong- yu, Li Yu- hong Cancer Center, Sun Yat- sen University of Medical Sciences, Guangzhou 510060,;Advanced and Recurrent Malignant Lymphoma Were Treated by BEAC Regimen Supported with Autologous Hemotopoietic Stem Cells Transplantation[J];Chinese Journal of Cancer;2001-04
3 WEI Zai shan, XU Xiao jun, NING Ping & XU Jin qiu (College of Environmental and Engineering, Kunming University of Science and Technology, Kunming 650093, China);RESEARCH AND ADVANCE IN WASTEWATER TREATMENT BY FLOTATION[J];Journal of Safety and Environment;2001-04
4 Chang Yaping 1,Wu Yazhen 2,Li Pingya 1,Song Yuzhen 1,Ming Yue 2,Liu Jinping,Huo Desheng 1 (1\ Basic School of Medical Sciences;2\ Department of Ophthalmology,The Second Clinical College,NBUMS) 〔;Experimental observation on the therapy of herpes simplex virus keratitis with G-Rg_3[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;2000-02
5 CAO Xia 1,GAO Yu fei *,LI Hong 1,YANG Hong 1,YANG Shi jie 1,DU Xue rong 2 (1 Dept.of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021 China; 2\ Department of Chinese Traditional Medicine,China Japan Union Hospit;Comparative study of effects of Ginsenosides,Panaquilons and Eleutherosides on isolated working heart[J];Journal of Norman Bethune University of Medical Science;2001-03
6 CHEN Sheng wu,WANG Yan,WANG Yi,WANG Li juan,HE Zhong mei,WANG Ben xiang (Jilin Institute of Natural Medicine,TianYao Science and Technology Co.,Ltd,Changchun 130012,China);Study on Anti-Tumor Activity of Ginsenoside Rg_1 and Rh_1[J];Journal of Norman Bethune University of Medical Science;2003-01
7 YU Xiao feng 1, QU Shao chun 1,XU Hua li 1,SUI Da yuan 1,CHEN Yan ping 2, MA Xing yuan 2 (1. Dept.of Pharmacology,Pharmacy College, Jilin University ,Changchun 130021, China; 2. Dept.of Chemistry ,Chemical College, Jilin University) [;Protective Effect of GFS on Experimental Myocardial Infarction in Rats[J];Journal of Jilin University of (Medicine Edition);2003-05
8 ;Application of foramen magnum absorption resin to extract saponins from American ginseng[J];Journal of Jilin University of (Medicine Edition);2004-05
9 WANG Qiu-jing~1, LIU Jie~1, LIU Fen~1,LI Yang~2(1. Experimental Center of Functional Sciences, School of Basic Medical Sciences, Jilin University,Changchun 130021,China;2. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University,Changchun 130021,China);Protective effects of PDS on acute cardiogenic shock in dogs[J];Journal of Jilin University of (Medicine Edition);2005-04
10 LIU Jin-ping, ZHAO Chun-fang, LI Ping-ya , LI Xian ( Department of Natural Medicine, Shenyang Pharmaceutical University, Shenyang 110015, China; Department of New Drug, Institute of Froniter Medical Sciences, Jilin University, Changchun 130021, China; Department of Pharmaceutical analysis, Jilin University, Changchun 130021, China);Ameliorative effects of pseudoginsenoside GQ on isoproterenol-induced acute myocardial ischemia in rats[J];Journal of Jilin University(Medicine Edition);2006-01
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 WANG Fang(Shaanxi Institute of Education,Xi'an,Shaanxi 710100);Preparation of Fresh American Ginseng Honey Processed Health Wine[J];Journal of Anhui Agricultural Sciences;2011-21
2 CAO Ying-hua,JIA Li,QIAN Chun-yan,XIA Min*,WANG Ying(Beijing Center for Physical and Chemical Analysis,Beijing 100089,China);Determination of Sugar Alcohols in by Liquid Chromatography with ELSD[J];Food Research and Development;2011-02
3 JIANG Shi-cui,LIU Wei-can,WANG Yi,SUN Chun-yu,LI Xiao-kun,ZHANG Mei-ping Engineering Research Center of Bioreactor and Pharmaceutical Development,Ministry of Education,Jilin Agricultural University,Changchun 130118,China;Correlation between ginsenoside accumulation and SQS and SQE gene expression in different organs of Panax quinquefolius[J];Chinese Traditional and Herbal Drugs;2011-03
4 YU Yan-ting,REN Li,SUN Chun-yu,JIANG Shi-cui,WANG Yi,ZHANG Mei-ping Cell Engineering Laboratory of Life Science College,Jilin Agricultural University,Changchun 130118,China;The construction and transformation of Ginseng SQE gene interference vector[J];Chinese Medicinal Biotechnology;2011-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wu GuangXuan; Wang Chunrong; Wei Yongdi; Ma Xingyuan, Xu Jingda(Department of Chemistry);High-performance liquid chromatographic analysis of the saponins in Panax quinquefolium[J];;1989-03
2 Li Giping; Yang Shijie; Liu Fen(Department of Pharmacology);Effect of PQS on the platelet aggregation rate and SOD activity in the hyperlipidemia rats[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1996-04
3 Guo Zhongyu,Zong Ruiyi ,Li Feng,et al (Dept.of Pathophysiology;  Dept.of Pharmacology);Study on effect of Antifatigue I against tiredness and anoxia[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1997-02
4 GAO Yong, WANG Jie Jun, XU Qing, YE Qin Qin, GUO Jing, GENG Huai Cheng (Department of Oncology, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China);Inhibitory effect of ginsenoside Rg3 on tumor neoangiogenesis[J];Academic Journal of Second Military Medical University;2001-01
5 Zhang Chongxi;STUDY ON THE CHEMICAL COMPOSITION OF AMERICAN GINSENG (PANAX QUINQUEFOLIUM)GROWN IN CHINA[J];Journal of Jilin Agricultural University;1988-01
6 MENG Xiang ying 1, LI Xiang gao 1, YU Yang 2 (1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118,China; 2. Changchun Medicine Group, Changchun 130021,China);Chemical Constituents of Flowerbuds of Panax quinquefolium L. Cultivated in China Ⅰ. Isolation, Identification and Content Determination of Saponins[J];Journal of Jilin Agricultural University;2000-03
7 Li Xianggao et al. (College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118);Chemical Composition from Fruit of Panax Quinquefolium[J];JOURNAL OF JILIN AGRICULTURAL UNIVERSITY;1998-02
8 Zhao Yanjie,et al(Pharmacology Dept., Jinzhou Medical College);COMPARATIVE STUDY ON THE STRENGTHENINGEFFECTS OF PANAX GINSENG(PG) AND PANAXQUINQUEFOLIUM(PQ)[J];JOURNAL OF JINZHOU MEDICAL COLLEGE;1998-03
9 Yang Yanwu, Xu Xiaolong, Qiu Jianqing, Wang Dehua and Qian Baogong (Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics, the Chinese Academy of Sciences) Ma Xingyuan and Xu Jingda ( Department of organic chemistry, bethune University of Medical science);NUCLEAR MAGNETIC RESONANCE STUDIES OF GINSENOSIDE——Ⅰ. Structure Elucidation of an Ocotillol-Type Saponin Separated From the Leaves of Panax Quinquefolium L.[J];Chinese Journal of Magnetic Resonance;1991-03
10 Li Yan; Qu Shaochun; Sun Wenjuan et al (Norman Bethune University of Medical Science,Changchun 130021 );Influence of Panax Quinquefolium Root Polysaccharide (PQRP) on Immunologic Function of Immunosuppressive Mice[J];Chinese Journal of Biologicals;1996-02
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