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《LISHIZHEN MEDICINE AND Materia MEDICA RESEARCH》 2008-04
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Preparation and RP-HPLC Analysis of Ursolic Acid from Paulownia tomentosa(Thunb.) Steud Leaves

YI Yan-ping1,ZOU Sheng-qin2,XIE Wan-bin1,CHEN Wu1,2* (1.College of Chemistry and Bioengineering of Yichun University,Yichun,Jiangxi 336000,China;2.Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun,336000,China)  
Objective:To extract and identify ursolic acid from Paulownia tomentosa(Thunb.) Steud leaves and to establish a determination method of ursolic acid.Methods:Ursolic acid was extracted from Paulownia tomentosa(Thunb.) Steud leaves by adopting a new method of ethanol extraction and agglutination separation,and its structure was identified by IR,MS and NMR.A determination method of ursolic acid in Paulownia tomentosa(Thunb.) Steud leaves by reversed-phase high performance liquid chromtograph was established.The chromatographic column(KromasilC18,4.6 mm×250 mm,5 μm),acetonitrile-methanol-water-phosphoric acid mobile phase(168:682:150:1.68,V/V) with 0.8 ml/min flow rate,the detected wavelength(210 nm) and the column temperature(25℃) were adopted.Results:Ursolic acid showed good linear relationship in the range of 0.92~16.56 μg(r=0.999 6).The average recovery rate was 99.82%(RSD was 0.75%).The content was 99.61% and the gain rate was 3.05 ‰.Conclusion:The method of ethanol extraction and agglutination separation to extract ursolic acid is advanced,rational,practical and feasible,and it provides technology condition for industrial production of ursolic acid.RP-HPLC is simple and accurate,it has good repeatability and wider linear range,and it can be used to evaluate the quality of ursolic acid.
【Fund】: 国家“863”计划重点资助项目(No.2002AA2Z3217)
【CateGory Index】: R284.2
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