Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Lishizhen Medicine and Materia Medica Research》 2008-10
Add to Favorite Get Latest Update

Study on the Extraction Technology and Determination of Flavones in Alfalfa

FENG Tao,BIAN Rui-huan,TANG Ran-xiao,LIU Hai-yan,WU Qiu-hua*,JI Shu-jing,LIU Shu-jing(College of Sciences,Agricultural University of Hebei,Baoding 071001,China)  
Objective To determine the content of flavonoids and study the extraction of Alfalfa.Methods Based on comparison of 3 kinds of heating reflux,Soxhlet' s extractor,ultrasonic extraction,with total flavonoids as index.the parameters of ultrasonic extraction on the basis of flavonoids were performed and comfirmed by orthogonal design.Results The order of factors which affected the flavonoid extraction was ratio of liquid to solidextraction timesextraction timeethanol concentration,and the best match was A3B2C1D1.Conclusion The optimum extraction conditions for Alfalfa flavonoids were established as follows: ultrasonic extraction,60% ethanol(v/v) as solvent,ratio of liquid to solid 30∶1(V/W),reflux extraction 3 times and 1 h for each time.The content of flavonoids in Alfalfa was 3.75%,and in leaves of Alfalfa was 4.85%.
【Fund】: 河北农业大学非生命学科科研发展基金资助项目
【CateGory Index】: R284.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 7 Hits
1 CAO Wei-guo~(1,2),LIU Zhi-qin~1,SHAO Yun~1,TAO Yan-duo~*(1 Northwest Institute of Plateau Biology, Chinese Academy of Sciences ,Xining 810001,China;2 Graduate School of the Chinese Academy of Sciences,Beijing 100031,China);A progress in pharmacological research of flavonoids[J];Acta Botanica Boreali-occidentalia Sinica;2003-12
2 JIANG Tingfu, SHI Yanpingaboratory for Natural Medicine of Gansu Province, Lanzhou730000, China);Analysis of Flavonoids in Natural Products with HPLC[J];Analysis and Testing Technology and Instruments;2002-04
3 ZHANG Dong-jie,XIA Mei-ru;Application of Nutrients of the Alfalfa to Functional Food[J];Journal of Heilongjinag August First Land Reclamation University;2002-04
4 CHEN Han-qing1,JIN Zheng-yu1,TAO Guan-jun2,QIN Fang2(1.College of Food Science and Technology,Southern Yangtze University,Wuxi 214036,China;2.Testing and Analysis Center,Southern Yangtze University,Wuxi 214036,China);Determination of Isoflavones in Different Parts of Trifolium pratenseby High Performance Liquid Chromatography[J];Food Science;2004-06
5 CHEN Han-qing,JIN Zheng-yu (School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China);Study on Extraction Technology of Trifolium pratense Isoflavones[J];Food Science;2005-04
6 ZENG Hong yan 1, ZHOU Pu hua 2, HOU Tuan zhang 3 (1 College of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan Hunan 411105, China; 2 Hunan University of Agriculture, Changsha Hunan 410128, China; 3 Institute of Hunan DS;Advances in studies on chemical constituents of Trifolium pratense[J];Chinese Traditional and Herbal Drugs;2001-02
7 BIAN Qing-quan 1, YANG Zhen-ping 2, MA Mei-zhong 3, LI Song 1 (1. Mianyang Teacher's College, Mianyang 621000, Sichuan, China; 2. Southwest University of Science and Technology, Mianyang 621000, Sichan, Chi na; 3. Sichuan University, Chendu 610065, Sichan, China);Study of the Methods of Determining the Isoflavones in Herbs of Trifolium pratense Extractive[J];China Journal of Chinese Materia Medica;2003-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Guo-feng,LI Lan-fang,ZHANG Qin-zeng,HAO Na,WANG Wei(Hebei Center for Disease Control and Prevention,Shijiazhuang 050021,China);Acute Toxicity and the Genotoxicity of Alfalfa Total Saponins[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-01
2 QIN En-hua (Bioscience and Technology College,Hubei Nationalities Institute,Enshi,Hubei 445000);Evaluation of the Content of Isoflavones Extracted from Trifolium pratense with RP-HPLC Method[J];Journal of Anhui Agricultural Sciences;2006-12
3 ZHANG Yue-lin (Agricultural Science Department of Dongying Vocational College,Dongying,Shandong 257091);Study on the Content & Extraction Technology of Flavonoid from Locust[J];Journal of Anhui Agricultural Sciences;2006-17
4 AN Shou-qiang et al (College of Agronomy,Northwest University of A & F Science and Technoloy,Yangling,Shaanxi 712100);Studies on Optimum Technology of Extracting Total Flavonoids from Tartary Buckwheat Seed by Microwave[J];Journal of Anhui Agricultural Sciences;2007-10
5 WANG Xin et al(Northwest A & F University,Yangling,Shaanxi 712100);Study on the Extraction Method of Flavonoid Compounds in Orychragmusviolaceus and its Antioxidation[J];Journal of Anhui Agricultural Sciences;2007-27
6 LI Qi-fan et al(School of Environmental Science and Engineering,Nanchang University,Nachang,Jiangxi 330031);Extraction and Purification of Drug Component-Total Isoflavones from Pueraria[J];Journal of Anhui Agricultural Sciences;2007-27
7 SHI De-fang et al (National Research and Development Center for Edible Fungi Processing,Agricultural Products Processing Subcenter of Hubei Agricultural Science and Technology Innovation Center,Research Institute of Agricultural Products Processing and Nuclear-Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064);Research Advances on Physiological Activity Function of Hesperidin and Its Extraction Technique[J];Journal of Anhui Agricultural Sciences;2008-10
8 XUE Xiao-li(Experimental Center,Jilin Academy of Agricultural Science and Technology,Jilin,Jilin 132101);Research on the Extraction Technology of the Total Flavonoids from Lotus Leaf[J];Journal of Anhui Agricultural Sciences;2009-12
9 ZHANG Ze-ying(Department of Biotechnology,Wuhan Bioengineering Institute,Wuhan,Hubei 430415);Optimization of Extraction Technology of Garlic Flavones by Orthogonal Test[J];Journal of Anhui Agricultural Sciences;2009-22
10 WANG Jing, HAN Tao,LI Li-ping (Beijing Agricultural College, Beijing 102206, China);Study and Applications of Ultrasound Wave in Biological and Food Technology[J];Journal of Beijing Agricultural College;2006-01
China Proceedings of conference Full-text Database 4 Hits
1 Li Fengying, Cui Ruijing, Zheng Lihong and Li Chunhua ( Department of Food Science, Hebei Normal University of Science & Technology, Changli Hebei 066600, China);Study on Contents of Flavonoid Compound and Proanto-cyanidins in Plants[A];[C];2004
2 ZHANG Rong-biao LIN Jian FAN Fu-rong (Fujian Center for Disease Control and Prevention,Fuzhou 350001,Fujian,China);The Effect on Blood-fat in Rats by Ganoderma Spores Propolis[A];[C];2008
3 WANG Zhan-bin1,DEI Yi-qiang1,ZHU Shi-kang1 Lifeng(1 College of Animal Science and Technology,Henan University of Science &Technology,Luoyang Henan 471003;2 NanyangHasbandry and Vet Station Nanyang Henan 473000);The Processing Parameters Optimization of the Flavonoids Extraction from the seeds of Robinia Persudoscacia in Microwave-assisted Extraction[A];[C];2008
4 WANG Yan-hua~(1,2),WANG Cheng-zhang~1,GAO Yong-ge~2,YAN Xue-bing~1,SHI Ying-hua~1,HE-Yun~3 (1.Engineering College of Animal Husbandry and Veterinary Science of Henan Agricultural University,Zhengzhou, 450002,China;2.Forage and Feed Station of Henan Province,Zhengzhou 450008,China;3.College of Animal Science of the Institute of Science and Technology,Xinxiang 453003,China);Effects of alfalfa saponins on the production performance and blood biochemical index of weaned piglets[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG RONG-QING HAN ZHENG-KANG CHEN JIE ZHANG CHONG -LI, (Lab of Animal Physiology and Biochemistry, Nanjing Agric. Univ., Nanjing 210095) (Institute of Zoology, Chinese Academy of Sciences, Beijing 100080);EFFECTS OF DAIDZEIN ON IMMUNE FUNCTION AND GH、PRL、SS LEVELS OF SERUM AND COLOSTRUM IN SOWS[J];Acta Zoologica Sinica;1995-02
2 TIAN Li-ping, LIU Qing-guang (College of Bio-Engineering, Shihezi University, Shihezi 832003, China);Development of Studies on Comprehensive Utilization and Processing Techniques of Medicago Sativa L.[J];Grass-feeding livestock;2004-02
3 WANG Jin feng 1,LIU Ling 2,ZHENG Yu cai 1,HE Ping 2 (1 Department of Life Science and Technalogy College,South west Nationality Colloge, Chengdu 610041,China;2 Liangshan Grassland Station,Xichang 615000,China);Technology for plant leaf protein extraction and utilization[J];Pratacultural Science;2003-01
4 LUO Fu cheng, CHEN Gong (Pratacultural Science Department, Yunnan Agricultural University, Kunming 650201, China);Fat-breeding effects of full-grazing turkey on regressed grassland[J];Pratacultural Science;2003-10
5 Jiang Yulin; Lu Xinshi;Shen Yulong; WangXi; LingFu; LiangLina(Lanzhou Institute of Animal Science , Chinese Academy of Agricultural Sciences, Lanzhou 730050);THE NUTRITION EVALUATION OF ALFALFA CULTIVARS IN CHINA[J];PRATACULTURAL SCIENCE;1995-02
6 Dong Kuanhu Jin Zongli Yang Guiying (Department of Animal Science, Shanxi Agricultural University, Taigu 030801) Xieben Long (Central Agricultural Test Farm of Hokkaido, Japan);A COMPARISON OF PRODUCTION PERFORMANCE BETWEEN NINE ALFALFA VARIETIES[J];PRATACULTURAL SCIENCE;1997-04
7 Zhang Shirun, Wang Lijuan, Liu Shouxin(Northeast Forestry University, Harbin 150040, P. R. China); Zhuang Qian(Forest Botanical Garden of Heilongjiang Province);The Optimum Extracting Conditions of Flavonoids in Hawthorn Leaves[J];Journal of Northeast Forestry University;2001-04
8 Zou Yaohong (Department of Chemistry, Changshu College, Changshu 215500) Li Guirong (Dongcheng Company of Domestic Chemistry, Zengcheng 511370);Analysis of Flavonoid as Antioxidant in Myrica Rubra Leaf With Reversed-phase High Performance Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;1998-05
9 LIU Zhen nan (Dept. of Chemistry and Chemical Engineering, Guangxi University for Nationalities,Guangxi Nanning,530006 China)$$$$;On the Alcoholic Extracting Technique of Flavonoids in Crataequs leaves by Using the Orthogonal Design[J];JOURNAL OF GUANGXI UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCE EDITION);1999-03
10 Zuo Xiongjun, Li Jing (Guangzhou Institute of Chemistry,Chinese Academy of Sciences,Guangzhou 510650);HPLC Analysis of G.B.L.Flavonoids in Extracts and Residue Leaf Extracted by SF-CO_2 with and without Entrainer[J];GUANGZHOU CHEMISTRY;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved