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《Lishizhen Medicine and Materia Medica Research》 2008-10
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Study on the Extraction Technology and Determination of Flavones in Alfalfa

FENG Tao,BIAN Rui-huan,TANG Ran-xiao,LIU Hai-yan,WU Qiu-hua*,JI Shu-jing,LIU Shu-jing(College of Sciences,Agricultural University of Hebei,Baoding 071001,China)  
Objective To determine the content of flavonoids and study the extraction of Alfalfa.Methods Based on comparison of 3 kinds of heating reflux,Soxhlet' s extractor,ultrasonic extraction,with total flavonoids as index.the parameters of ultrasonic extraction on the basis of flavonoids were performed and comfirmed by orthogonal design.Results The order of factors which affected the flavonoid extraction was ratio of liquid to solidextraction timesextraction timeethanol concentration,and the best match was A3B2C1D1.Conclusion The optimum extraction conditions for Alfalfa flavonoids were established as follows: ultrasonic extraction,60% ethanol(v/v) as solvent,ratio of liquid to solid 30∶1(V/W),reflux extraction 3 times and 1 h for each time.The content of flavonoids in Alfalfa was 3.75%,and in leaves of Alfalfa was 4.85%.
【Fund】: 河北农业大学非生命学科科研发展基金资助项目
【CateGory Index】: R284.2
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