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《Lishizhen Medicine and Materia Medica Research》 2010-01
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Extraction and Preparation of Crude Toxin from Argiope amoena

CHEN Lian-shui1,ZHANG Hao2,YUAN Feng-hui1,MAO Yue-xiang2,CAO Ning2,ZHU Mang3(1.Department of Biology,East China Institute of Technology,Fuzhou 344000,China;2.The Junior of Department of Biology,East Chian Institute of Technology,Fuzhou 344000,China;3.The Junior of Department of Biology,East Chian Institute of Technology,Fuzhou 344000,China)  
Objective Having a detailed mastery about the Argiope amoena' s morphological characteristics and living habits,we analyzed the relevence of poison gland weight and length to its body.Using the optimized method we obtained higher biological properties and more pure venom.Methods We got the relevence of poison gland weight and length to its body using statistical method by anatoming poison gland.Isolating poison gland from spider,obtained stable and maximum amount of venom,we tested titer of venom and explore the type of inhibit ion channels toxin using chicken red blood cells as a model.Results Venom gland' s weight and body mass were positively correlated.With the optimized mean prepared method,five millingrams of crude venom were got.The venom has effect of promoting red blood cell aggregating and cracking.The blood cell cracked more seriously in the Calcium ions environment.Conclusion More qualified venom can be got by venom gland dissection.We initially know that venom contains phospholipase lectin and agglutinin.
【Fund】: 国家自然科学基金(No.30370208);; 东华理工大学学生科技创新基金资助项目
【CateGory Index】: R284.2
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