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《Lishizhen Medicine and Materia Medica Research》 2011-06
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Comparative Study on Polysaccharide from Sipunculus nudus by Different Extraction Process

LIU Yu-ming1,QIAN Tian-tian1,HE Yin1,JIANG Ding-wen1,SHEN Xian-rong1*,JIANG Shu-qi2,YANG Jin-gang3(1.Naval Medical Research Institute,Shanghai,200433,China;2.Zhejiang Traditional Medicine University,Zhejiang Hangzhou,310053,China;3.Pharmacological University,Nanjing,211198,China)  
Objective To study enzyme treatment,alkali treatment and a combination of the two processes for the extraction of Sipunculus nudus polysaccharide and compare these three methods.Methods The content of polysaccharide was determined by colorimetry using anthrone-sulfuric acid method,and the yield of polysaccharides was obtained by gravimetric method.Results The results indicated that the yield of polysaccharides was 0.60%(enzyme method),1.47%(alkali method) and 0.20%(combined method),and the content of polysaccharide was 21.83%、87.30% and 36.40% respectively.Conclusion The extraction process of alkali method is feasible and is more efficient than the other two methods.
【Fund】: 国家科技重大专项新药创利项目(No.2008ZXJ09004-027)
【CateGory Index】: R284.2
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