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《Lishizhen Medicine and Materia Medica Research》 2011-06
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Comparative Study on Polysaccharide from Sipunculus nudus by Different Extraction Process

LIU Yu-ming1,QIAN Tian-tian1,HE Yin1,JIANG Ding-wen1,SHEN Xian-rong1*,JIANG Shu-qi2,YANG Jin-gang3(1.Naval Medical Research Institute,Shanghai,200433,China;2.Zhejiang Traditional Medicine University,Zhejiang Hangzhou,310053,China;3.Pharmacological University,Nanjing,211198,China)  
Objective To study enzyme treatment,alkali treatment and a combination of the two processes for the extraction of Sipunculus nudus polysaccharide and compare these three methods.Methods The content of polysaccharide was determined by colorimetry using anthrone-sulfuric acid method,and the yield of polysaccharides was obtained by gravimetric method.Results The results indicated that the yield of polysaccharides was 0.60%(enzyme method),1.47%(alkali method) and 0.20%(combined method),and the content of polysaccharide was 21.83%、87.30% and 36.40% respectively.Conclusion The extraction process of alkali method is feasible and is more efficient than the other two methods.
【Fund】: 国家科技重大专项新药创利项目(No.2008ZXJ09004-027)
【CateGory Index】: R284.2
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Chinese Journal Full-text Database 4 Hits
1 SHEN Xian rong, JIANG Ding wen, JIA Fu xing, et al. ( Marine Biology Research Center, Naval Medical Research Institute, Shanghai 200433, China);Study on anti-senescence effect of Sipunculus nudus preparation[J];Chinese Journal of Marine Drugs;2004-01
2 SHEN Xian-rong,JIANG Ding-wen,LU Min,CHEN Wei,LUAN Jie,YANG Yifang,ZHANG Jian-guo(Naval Medical Research Institute,Shanghai 200433,China);Protective effect of Sipunculus nudus preparation on mouse irradiated by ~(60)Co[J];Chinese Journal of Marine Drugs;2008-02
3 HOU Bao-lin,WANG Xi,Hai lixi·taoerdahong(Xinjiang Medical University,Xinjiang 830054,China);Research on Polysaccharide Extraction and the Content of Brassica rapa L.[J];Lishizhen Medicine and Materia Medica Research;2009-11
4 SHEN Xian-rong, JIANG Ding-wen, JIA Fu-xing, et al.Naval Medical Research Institute, Shanghai 200433, China;The anti-fatigue effect of Sipunculidae preparation on mice[J];Chinese Journal of Nautical Medicine;2003-02
Chinese Journal Full-text Database 10 Hits
1 SU Xiu-Rong1**, JI Qi-Xiong2, DING Li-Fa3,ZHANG Wei4, ZHU Jun-Quan1, LI Tai-Wu11.Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211,Zhejiang,China2.Applied Biology Department of Zhejiang Pharmceutical College,Ningbo Zhejiang 315000, China3.Wenzhou Fisheries Technical Extension Station, Wenling 317500,Zhejiang,China4.Colloge of Biology, Liaoning Normal University,Dalian Liaoning 116029, China;Microstructure and ultrastructure of coelomocytes of the sipunculan Phascolosoma esculenta[J];Acta Zoologica Sinica;2008-01
2 HUANG Xiao-Chun, SU Xiu-rong, SU Yue-ping(Faculty of Life Science and Technology, Ningbo University, Ningbo 315211, China);Approximate Compositions of Perinereis aibuhitensis and Phasolosma esculenta[J];Fisheries Science;2005-06
3 DENG Zhong-ri1,HUANG Bo2,FANG Zai-guang2(1.Department of Chemistry and Environmental Engineering,Hunan City College,Yiyang 413000,China;2.Ocean College,Hainan University,Haikou 570228,China);Morphological and Histological Characteristics of Body Wall in Phscolosoma esculenta[J];Fisheries Science;2007-06
4 SONG Hong-xin,CHUAN Juan-juan,LIU Jing(Life Science & Engineering College,Shaanxi University of Science and Technology,Xi'an 710021,China);Resveratrol Extraction from Polygonum cuspidatum with Enzymic Dissolution[J];Acta Botanica Boreali-Occidentalia Sinica;2010-12
5 Yang Shengdan,Fu Dayou(Material and Chemical Engineering College,Sichuan University of Science and Engineering,Zigong 643000,China);Application of Ultrasonic Wave, Microwave-assisted Extraction and Their Combined Extraction Technology of Effective Constituents from Chinese Herbal Medicine[J];Guangdong Chemical Industry;2010-02
6 LI Ke-xian,SHEN Xian-rong,JIANG Ding-wen,HE Ying,YIN Ming,LIN Meng,LIU Dai-hao,LIU Yu-ming(Naval Medical Research Institute,Shanghai 200433,China);Comparision of the nutritive components in Sipunculus nudus from three different places of Chinese coastal areas[J];Journal of Navy Medicine;2010-01
7 DENG Zhong-ri, HUANG Bo, FANG Zai-guang (Department of Chemistry and Environmental Engineering, Hunan City University, Yiyang, Hunan 413000, China; Ocean College, Hainan University, Haikou 570228, China );Morphological and Histological Characteristics of the Body Wall in Sipunculus Nudus[J];Journal of Hunan City University(Natural Science);2007-01
8 DENG Zhong-ri 1a,HUANG Bo 2,KU Wen-zhen 1b(1a.Department of Chemistry and Environmental Engineering;1b.Department of Computer Science,Hunan City University,Yiyang,Hunan 413000;2.Ocean College,Hainan University,Haikou,Hainan 570228,China);Morphology and Histological Structure of the Caecum in Sipunculus Nudus Linnaeus,1766[J];Journal of Hunan City University(Natural Science);2009-02
9 WEI Jing-na~1,ZHU Hong-ji~1,LIN Qiang~2(1.School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China;2.Biochemical Engineering College,Beijing Union University,Beijing 100023,China);Extraction of Polysaccharides from Stigma maydis by Enzyme and Ultrasound[J];Chemical Industry and Engineering;2008-02
10 ;Advances on research of Sipunculus[J];Marine Sciences;2008-06
China Proceedings of conference Full-text Database 2 Hits
1 Yu hua1 Chen Fang2*(1.Sichuan Entry-Exit Inspection and Quaranting Bureau of P.R.China,Sichuan Chengdu,610041;2.School of Pharmaceutical sciences,Southwest University,Chong qing,400715,China);Study on the Primary Process of Glycyrrhizic Acid Extracting from Glycyrrhiza[A];[C];2008
2 Li Xue,Guo Yan-xia,Ren Hui-xia(Department of Pharmacy,Shandong University.Ji Nan,Shandong,China);Recent review in extraction techniques and pharmacological study of Baicalin and Baicalein[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XU Wei1, ZHENG Hai-yin2, ZHENG Xiao-yan2, et al (1. Department of pharmacy, FUTCM, Fuzhou, Fujian 350108, China; 2. Department of Integrative Medicine, FUTCM, Fuzhou, Fujian 350108, China);Determination of Polysaccharides in Salvia Chinensis by Anthranone-sulfuric Acid and Constituents Analysis[J];Journal of Fujian University of Traditional Chinese Medicine;2008-01
2 Huang Yuliang Huang Qun Huang Zheyuan,et al(Dept. of Modern Medicine, FCTCM);Effect of Fufangxingchong Koufuye to Immunologic Function in Mice[J];JOURNAL OF FUJIAN COLLEGE OF TRADITIONAL CHINESE MEDICINE;1998-02
4 SHEN Xian rong, JIANG Ding wen, JIA Fu xing, et al. ( Marine Biology Research Center, Naval Medical Research Institute, Shanghai 200433, China);Study on anti-senescence effect of Sipunculus nudus preparation[J];Chinese Journal of Marine Drugs;2004-01
5 FAN Zhi Gang ①,LI Yu Lian,YANG Li Bin ②,KUANG Li Ping ③ ① Wulumuqi General Hospital,Lanzhou Command of PLA.Wulumuqi 830054,Xinjiang ② Pharmaceutical College,Xinjiang Medical University.Wulumuqi 830000,Xinjiang;Influence of Microwave Technique on the Amount of rutin Diffused from Sophora Flower[J];PHARMACEUTICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY;2000-01
6 Chen Meng, Yuan Dongxing, Xu Pengxiang(Environmental Science Research Center, Xiamen University, Xiamen 361005);Research Progress on Microwave Extraction[J];JOURNAL OF INSTRUMENTAL ANALYSIS;1999-02
7 Yuan Ke,Liu Yanze,Ji Chunru (Henan college of Traditional Chinese Medicine,Zhengzhou 450003);THE COMPARISION AMONG THE SEVERAL DIFFERENT EXTRACTION METHODS IN THE RESEARCH OF THE CHEMICAL COMPONENTS OF CHINESE MEDICINAL PLANTS[J];Natural Product Research and Development;1997-04
8 TU Fang,YANG Fang,ZHENG Wen-jie*,BAI Yan(Department of Chemistry,Jinan University,Guangzhou 510632,China );Progress in Polysaccharide of Spirulina[J];Natural Product Research and Development;2005-01
9 Yuan Ke(Yuan K),Jian Xiumei(Jian XM),Meng Jiang(Meng J)(Henan College of Traditional Chinese Medicine,Zhengzhou 450003);Compaprision among several different extraction methods for Herba plantaginis[J];CHINESE JOURNAL OF MODERN APPLIED PHARMACY;1999-04
10 Ma Long, Deng Shuwen, Qu Weidong, et al Department of Environmental Hygiene, Xinjiang Medical College;Experimental Research on the Antiaging Effects of Xinjiang Turnip[J];Journal of Xinjiang Medical University;1998-01
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