Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Lishizhen Medicine and Materia Medica Research》 2012-02
Add to Favorite Get Latest Update

Observation on the Analgesic Effect of Repeated Electroacupuneture and Its Relation to the Expression of CAMKⅡ in Rat Hypothalamus

XU Qiu-ling1, CHEN Shu-ping2, GAO Yong-hui2, WANG Jun-ying2, LIU Jun-ling2(1.Hainan Medical University,Haikou 571101,China;2.Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)  
Objective To observe the accumulative analgesic effect of electroacupuncture(EA) and its relation to the expression of Calcium/calmodulin-dependent protein kinaseⅡ(CAMKⅡ) in rat hippocampus and hypothalamus tissues in chronic constrictive injury(CCI) and ovariectomy(OVX) rats. Methods A total of 70 Wistar rats were randomized into control,CCI,CCI+EA 2 day(D),CCI+EA 2 week(W),OVX+CCI,OVX+CCI+EA2D and OVX+CCI+EA2W groups,10 cases in each group.For rats of the latest 3 groups,subcutaneous injection of D-galactose was given once daily for 5 w.Morris water maze test was conducted to evaluate the memory-learning ability.Chronic pain model was established by ligating the left sciatic nerve with a piece of catgut.EA(2/15Hz,1-2 mA) was applied to bilateral "Zusanli"(ST 36)-"Yanglingquan"(GB 34),for 30min,once daily for 2 d,2 w respectively.Radiant heat irradiation-induced paw withdrawal latency(PWL,pain threshold) was detected and PWL difference values between two paws were used as the hyperalgesia score(HAS).CAMKⅡ expression in paraventricular nucleus(PVN) of hypothalamus was detected by immunohistochemistry. Results Compared with control group,HAS was increased significantly after CCI.HAS in CCI+EA2W group was markedly lower than that of CCI and CCI+EA2D groups(P0.05).In comparison with OVX+ CCI +EA2W group,HAS in OVX+CCI+EA2W group was significant(P0.05).Interga(grey value igu of)CAMKⅡ immunoreaction positive product increased in rat hypothalamic PVN of CCI+EA2W group was significantly lower than that of CCI group(P0.05).IAV of CAMKⅡ is hypothalamus of OVX+CCI+EA2W group was significantly lower than that of OVX+CCI group(P0.05) but obviously higher than that of CCI+EA2W group(P0.05). Conclusion Repeated EA(2W) has an accumulative analgesic effect in both CCI and CCI+OVX rats,which is closely related to the upregulation of CAMKⅡ expression.OVX+D-galactose administration may weaken the cumulative effect of EA.
【Fund】: 国家自然科学基金重大研究计划(No.90709031);; 海南省自然科学基金(No.310054)
【CateGory Index】: R245
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 XU Qiu-ling,LIU Tao. Hainan Medical School,Haikou 571101,China;Therapeutic Evaluation of the Accumulative Analgesic Effect of Electroacupuncture[J];Shanghai Journal of Acupuncture and Moxibustion;2013-04
2 XU Qiu-ling,LI Jian-qiang,LIU Tao(Hainan Medical University,Haikou 571101,China);Analgesic Cumulative Effect of Electroacupuncture Based on Pain Index[J];Journal of Clinical Acupuncture and Moxibustion;2013-01
【Citations】
Chinese Journal Full-text Database 5 Hits
1 DONG Zhi-qiang1, MA Fei1, DA Cui-di 1,2, XIE Hong1, LI Wei-min1, WANG Yan-qing1, WU Gen-cheng1. 1. Department of Integrative Medicine, Institute of Acupuncture Research, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China;Cumulative Electroacupuncture Enhances Expression of GDNF mRNA in Dorsal Root Ganglions of Neuropathic Pain Rats[J];Shanghai Journal of Acupuncture and Moxibustion;2005-02
2 LUO Fei(Department of Physiology,Beijing Medical University,Beijing 100083);A Study on the Cumulative Effect of Repeated Electroacupuncture on Chronic Pain[J];PROGRESS IN PHYSIOLOGICAL SCIENCES;1996-03
3 LIU Jun-ling,CHEN Shu-ping,GAO Yong-hui,MENG Fan-ying,WANG Jun-ying(Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China);Observation on the Analgesic Effect of Repeated Electroacupuncture and Its Relation to Changes of Plasma β-EP,ACTH and COR Levels[J];Acupuncture Research;2007-05
4 XU Qiuling,CHEN Shuping,GAO Yonghui,et al.College of Acu-moxibustion,Beijing University of Chinese Medicine,Beijing,100029;Observation on the analgesic effect of repeated electroacupuncture and its relation to the expression of synaptophysin in rat's hippocampus and hypothalamus[J];Chinese Journal of Rehabilitation Medicine;2009-06
5 SUN Rui qing, WANG He chun, WANG Yun, et al . (Neuroscience Research Institute, Peking University, Beijing 100083,China);EFFECT OF ELECTROACUPUNCTURE WITH DIFFERENT FREQUENCIES ON NEUROPATHIC PAIN IN A RAT MODEL[J];Chinese Journal of Applied Physiology;2002-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Yu-hui, MA Wu-hua,GAO Xiao-qiu, et al. Department of Anesthesiology, First Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China;Comparison of the efficacy of acupuncture-assisted anesthesia with different frequency stimulation for thyroid surgery[J];Guangdong Medical Journal;2008-08
2 WANG Zhifu 1,YU Xiangmei 1,YAO Ranran 2,et al(1.College of Integrative Medicine,FUTCM,Fuzhou,Fujian 350122,China; 2.Shanghai Medical College,Fudan University,Shanghai 200032,China);Applied Anatomical Study on Surface Location of SD Rats Sciatic Nerve and Related Acupuncture Points[J];Journal of Fujian University of Traditional Chinese Medicine;2012-06
3 HUANG Shu-Qi,XIE Xue-Wei,ZHAO Zheng-Qing,SHAO Fu-Yuan Department of Neurology,Changzheng Hospital,Shanghai 200003,China;Effect of Erythropoietin on Neuron Apoptosis in Hippocampus of Vascular Dementia Rats[J];Chinese Journal of Clinical Neurosciences;2012-03
4 WANG Jun-ying,MENG Fan-ying,CHEN Shu-ping,GAO Yong-hui,LIU Jun-ling(Department of Physiology,Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China);Correlation between the Cumulative Analgesic Effect of Electroacupuncture and the Expression of Hypothalamic Pre-proopiomelanocortin mRNA and Hypothalamic and Hippocampal Prepronociceptin mRNA in Chronic Neuropathic Pain Rats[J];Liaoning Journal of Traditional Chinese Medicine;2011-03
5 WANG Nian-hong1,LI Jun2,PEI Ming3 (1.Yueyang Hospital of Integrative Chinese & Western Medicine Attached to Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;2. Gansu College of Traditional Chinese Medicine,Lanzhou 730000,Gansu,China;3. Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China);Analgesia Effect of Tuina on Pain of Experimental Rheumatoid Arthritis Rabbits[J];Journal of Liaoning University of Traditional Chinese Medicine;2007-06
6 LI Jun-han,Huang Lei 1.The sports and Healthy Education College of Mianyang Normal University,Sichuan Mianyang,621000; 2.The 3rd Ward Orthopedics Hospital of Sichuan Province,Sichuan Chengdu,610041,China.;The Experimental Study of the Prevention and Cure on Exercise-induced Fatigue in Rats by Different Frequency Electro-acupuncture at Zusanli[J];Sichuan Sports Science;2011-04
7 LI Hui 1, LI Xiao hong 2 1. Department of Acupuncture, Sino Japan Friendship Hospital, Beijing 100029, China; 2. Acupuncture School, Beijing University of Traditional Chinese Medicine, Beijing 100029, China;Research Progress of Neuropeptide and Acupuncture Immunoregulation[J];Shanghai Journal of Acupuncture and Moxibustion;2003-03
8 LI Yi1,JU Zi-yong1,XIA Yong1,LIU Shi-min1,SHI Xiao-lan2,HE Jin-sen1.1.School of Acupuncture-moxibustion and Massotherapy,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;2.Department of Acupuncture and Moxibustion,Shanghai Jiangwan Hospital,Shanghai 200434,China;Study of the Effect of Electroacupuncture in a Rat Model of Perimenopausal Depression[J];Shanghai Journal of Acupuncture and Moxibustion;2010-10
9 SHOU Yin1 XU Ming-shu2 GE Lin-bao3,4 1.Graduate School of Shanghai University of TCM,Shanghai 201203,China;2.Brain Neurobiology Laboratory,Shanghai Research Institute of Acupuncture and Meridians;3.Shanghai Research Institute of Qigong;4.Shanghai Research Center of Acupuncture and Meridians;Progresses of studies on acupuncture and pain signals transduction mechanisms in complete Freund's adjuvant arthritis[J];世界针灸杂志(英文版);2009-03
10 Junying Wang, Junling Liu, Shuping Chen, Yonghui Gao, Fanying Meng, Lina Qiao Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China;Acupuncture effects on the hippocampal cholinergic system in a rat model of neuropathic pain[J];中国神经再生研究(英文版);2012-03
China Proceedings of conference Full-text Database 1 Hits
1 WANG Nian-hong1 LI-Jun2 PEI-Min2 (1 Yueyang hosipital of Tranditional Chinese & western medcine,Shanghai University of TCM;2 GanSu College of TCM);Effect of Tuina Reliefing Pain on Experimental Rheumatoid Arthritis Rabbits[A];[C];2006
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 XIE Heng-hui,ZHEN Bin.Acupuncture-moxibustion Department,Beijing Chaoyang Hospital Affiliated to Capital Medical University,Beijing 100020,China;Observations on the Efficacy of Point Weizhong Blood-letting Puncture and Cupping Plus Acupuncture for Treating 50 Sciatica Patients[J];Shanghai Journal of Acupuncture and Moxibustion;2007-03
2 LIU Jun-ling,CHEN Shu-ping,GAO Yong-hui,MENG Fan-ying,WANG Jun-ying(Institute of Acu-moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China);Observation on the Analgesic Effect of Repeated Electroacupuncture and Its Relation to Changes of Plasma β-EP,ACTH and COR Levels[J];Acupuncture Research;2007-05
3 LUO Ai-lin, JIN Xiao-gao, ZHANG Guang-xiong,et al. Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;The change in PSD95 mRNA expression in spinal cord in a rat model of neuropathic pain[J];Chinese Journal of Anesthesiology;2005-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YU Shu-guang, LUO Song, HAN Ting, TANG Yong. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;Effect of electroacupuncture therapy on morphological synaptic plasticity in hippocanipal neurons of rats with senile dementia[J];Chinese Journal of Neuromedicine;2006-04
2 LIU Zhao ping, LI Wen xian, YU Bo, LU Cheng qian, CHEN Jun shi (Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China);Effects of estrogen-like herb extracts on estrogen receptors and β-endorphin in ovariectomized rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-14
3 ZHANG Lu-fen1,YAN Ming-ru1,CHENG Jin-lian2,et al(1 School of Acupuncture and Moxibustion,Beijing University of Chinese Medicine,Beijing 100029;2 Beijing Hospital of Traditional Chinese Medicine);Effect of acupuncture on β-EP levels of plasma,hypothalamus, pituitary and lymph node in rats with adjuvant-induced arthritis[J];Journal of Beijing University of Traditional Chinese Medicine;2007-01
4 DONG Zhi-qiang1, MA Fei1, DA Cui-di 1,2, XIE Hong1, LI Wei-min1, WANG Yan-qing1, WU Gen-cheng1. 1. Department of Integrative Medicine, Institute of Acupuncture Research, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China;Cumulative Electroacupuncture Enhances Expression of GDNF mRNA in Dorsal Root Ganglions of Neuropathic Pain Rats[J];Shanghai Journal of Acupuncture and Moxibustion;2005-02
5 LuXinhua(BaoSteelHospitalAfiliatedtoShanghaiSecondMedicalUniversity,201900);ANALYSISONTREATMENTOFSCIATICABYACUPUNCTURE[J];SHANGHAI JOURNAL OF ACUUNCTURE AND MOXIBUSTION;1998-02
6 Dong Hong-tao FANG Yi-gongNanjing University of Tradional Chinese Medicine,The First Hospial of Zibo City,Shandong;Establishment of Multi-factor AD Animal Model and Acupuncture Effect on Its Memory[J];Shanghai Journal of Traditional Chinese Medicine;2002-05
7 LUO Fei(Department of Physiology,Beijing Medical University,Beijing 100083);A Study on the Cumulative Effect of Repeated Electroacupuncture on Chronic Pain[J];PROGRESS IN PHYSIOLOGICAL SCIENCES;1996-03
8 Hu Sanjue Sha Jianhui Gu Jianven Han Yong (Department of Physiology,The Fourth Military Medical University,Xian 710032);Peripheral Antinociception of Acupunoture on Inflamed Hyperalgesia[J];Acupuncture Research;1991-Z1
9 Han Jisheng (Neuroscience Research Institute, Peking University, Beijing, 100083;New Evidence to Substantiate the Frequency Specificity of Acupuncture-induced Analgesia[J];Acupuncture Research;2001-03
10 YANG Xianghong, WANG Yanqing, GAO Xiu, WU Gencheng (Institute of Acupuncture Mechanisms, Department of Neurobiology, State Key Laboratory of Medical Neurobiology, Medical Center of Fudan University, Shanghai, 200032);Effects of Electroacupuncture on Hyperalgesia Score of Neuropathic Pain Rats[J];Acupuncture Research;2002-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved