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《Lishizhen Medicine and Materia Medica Research》 2012-12
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Cellulose Enzyme Extraction of Polysaccharide from Allium macrosttemon B.

XU Miao1,ZHENG Ru-gang1,LIANG Zhen-yi1,LIN Zhao-hua1,DENG Shi-ming2,CHEN Yi-ping1*(1.Key Laboratory of Ministry of Education for Application Technology of Chemical Materials in Hainan Superior Resources,Hainan University,Material&Chemical Engineering College,Haikou 570228,China;2.Ocean College,Hainan University,Haikou 570228,China)  
Objective To study the enzymatic extraction of polysaccharide from Allium macrostemon Beg.Methods With single factor test and L9(34) orthogonal experiment,the extraction technology of A.macrosttmon polysaccharide by cellulose enzyme method was studied.Results Optimum temperature was 50℃,time was 2.5 h,pH was 5.0,enzyme dosage was 0.4%,under these conditions,the extraction rate of A.macrostemon polysaccharides was 17.27%,and the rate of enzyme extraction was 34.19% more than the water extraction.Conclusion The cellulose enzyme extraction is beneficial to increase the extraction rate of A.macrostemon polyasccharides.
【Fund】: 国家科技服务企业项目(No.2009GJE20050);; 海南省重点科技计划项目(No.ZDXM20100041)
【CateGory Index】: R284.1
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