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《Lishizhen Medicine and Materia Medica Research》 2013-06
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The Isolation and Purification of oligopeptides from Ruditapes Philippinarum and its Inhibition on the Growth of PC-3 Cells in Vitro

XU Lv,YANG Zui-su,HUANG Fang-fang,LI Rong,WANG Xian,SUN Yu(School of Food Science and Pharmacy of Zhejiang Ocean University,Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products,Zhoushan 316004,China)  
Objective To study methods to isolate and purify oligopeptides from Ruditapes philippinarum(RPO) and its antitumor activity against PC-3.Methods Trypsin proteases were applied to hydrolyze the R.philippinarum.Ultrafiltration and anion-exchange chromatography and RP-HPLC were performed to separate and purify the antitumor peptides.MTT assay was used to detect the inhibition effect of the RPO against PC-3 in vitro.AO/EB and Annexin V-FITC/PI was used to detect the apoptosis on PC-3.Results RPO's molecular weight is 607.6Da.MTT assay showed that the purified RPO inhibited proliferation of PC-3 cells in both concentration-and time-dependent manners.Flow cytometry studies showed exposing PC-3 cells to RPO for 24h increased the percentage of the early apoptotic cells and cellular necrosis in a dose-dependent manner.In addition,typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining.Conclusion RPO can inhibit the growth of PC-3 cells and induce apoptosis.
【Fund】: 浙江省自然科学基金(No.LY12C20008)
【CateGory Index】: R285.5
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