Bio-imprinting of protein-nucleic acid mixture and its catalytic behavior in hydrolysis

CUI Ying1,CHU Qi1,YAO Lin1,HUANG Ji-tao1,2 (1.School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300191,China;2.College of Chemistry,Nankai University,Tianjin 300071,China)  

As a development of molecular imprinting technique,bio-imprinting in which proteins acts as matrix plays an important role in preparing enzyme mimics.Here,we use N-benzoyl-L-arginine ethyl ester as template to bio-imprint protein-nucleic acid mixture from herring sperm,and then catalyze the hydrolysis of N-benzoyl-L-arginine ethyl ester.The result shows that the bio-imprinted protein-nucleic acid mixture is stable in aqueous medium,thereby mimicking hydrolytic enzymes.

【Key Words】： bio-imprinting protein-nucleic acid mixture catalyzed hydrolysis active site
【CateGory Index】： Q503

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