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《Journal of Instrumental Analysis》 1993-01
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Application of Membrane Affinity Chromatography in the Sepatation of Trypsin Inhibitor

Shang Zhenhua;Yu Yinian;Guo Wei;Zhou Liangmo Dalian Institute of Chemical Physics, Academia Sinica  
Ultrafiltration membrane with large pores was prepared from chemically modified polysul-fone (CMPS) which were synthesized by modification of polysulfone (PS) with reactions ofacrylation-amination and chloromethylation-amination respectively, by phase reversion. Afterdiazotisation, trypsin was covalently coupled onto the CMPS membrane under pressure driving.The immobilized trypsin has a specific activity up to 10200 u/g, namely 15mg trypsin wasimmobilized on 1 g or CMPS membrane. The immobilized enzyme membrane can be used toseparate trypsin inhibitor and 6.5mg pure inhibitor was obtained in a run.
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【References】
Chinese Journal Full-text Database 9 Hits
1 CHAI Hong, QIAN Hua, CHEN Huanlin(College of Materials Science & Chemical Engineering, Zhejiang University, Hangzhou 310027, China);Affinity membrane separation and its development[J];膜科学与技术;2003-04
2 CHAI Hong, CHEN Huan-lin (College of Chemical and Material Engineering, Zhejiang University, Hangzhou 310027, China);Kinetic Model of Affinity Membrane Adsorption for Biomolecules in Stacked Flat-sheet Module[J];高校化学工程学报;2000-02
3 ZHENG Yu, CHEN Huan Lin *, LI Jing, CHAI Hong, LIU Mo E [WT5”BX](College of Chemical Engineering, Zhejiang University, Hangzhou 310027, China) [WT5HZ];Studies on the Affinity Membrane for Adsorption and Purification of Lysozyme(Ⅰ) Influence of Low temperature Oxygen Plasma Treatment on Surface Structure and Adsorption Properties of the Affinity Membrane[J];高等学校化学学报;1999-08
4 LI Jing, CHEN Huan Lin *, CHAI Hong (College of Chemical Engineering, Zhejiang University, Hangzhou 310027, China);Studies on the Affinity Membranes for Adsorption and Purification of Lysozyme(Ⅱ) Adsorption Characteristics of Metal Chelating Affinity Membranes for Lysozyme[J];高等学校化学学报;1999-08
5 Gan Hongyu , Shang Zhenhua, Wang Junde ( Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Dalian 116012);Recent Advances in Affinity Membrane Chromatography[J];分析化学;1999-01
6 Wang Jinfang, Meng Zihui, Zhou Liangmo, Wang Qinghai and Zhu Daoqian (Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, 116012);Development and Applications of High Performance Membrane Chromatography[J];色谱;1998-05
7 Wu Yanhui, Wang Shichang (Chemical Engineering Research Center, Tianjin University, Tianjin 300072);New Advances in Affinity Membrane Separation[J];化工时刊;1997-08
8 Shang Zhenhua; Go wei; Yu Yinian; Zhou Liangmo(Dalian Institute of Chem.Phys,Academia Sinica,Dalian 116012);Impurity Removal of Human Serum Albumin by Affinity Membrane Chromatography with Chemically Modified Cellulose[J];分析测试学报;1995-04
9 Shang Zhenhua;Zhou Liangmo(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116012);Current Situation,Development and Applications of Membrane Affinity Chromatography[J];化学进展;1995-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YU Ji, CAN Hong-Yu, HE Yi, YUAN Jim, CHEN Huan-Lin (College of Materials Science and Chemistry Engineering, ZheJiang University, Hangzhou 310027, China);Studies on PVDF Hollow Fiber Affinity Membrane for Separation of X-Globulin from Human Plasma( Ⅰ ) -Preparation of PVDF Hollow Fiber Affinity Membrane and Its Adsorption Properties[J];高等学校化学学报;2003-05
2 WEI Gui-lin, LIU Xue-liang, LI Jing-hua, LIU Ying, SHANG Zhen-hua (Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China);Endotoxin Removal in Some Medicines and Human Serum Albumin Solution by Affinity Membranes[J];色谱;2002-02
3 LI Jing, CHEN Huanlin, CHAI Hong (College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027,China);Studies on the affinity membrane for adsorption and purification of lysozyme (Ⅲ) New method of purificating lysozyme from enzyme mixture and egg white (shell)[J];膜科学与技术;2001-04
4 CHAI Hong, CHEN Huanlin and XU Li (College of Chemical Engineering, Zhejiang University, Hangzhou?310027,Zhejiang,China);IMMOBOLIZED METAL ION AFFINITY MEMBRANE PREPARED BY PLASMA-INDUCED GRAFT POLYMERIZATION AND ITS ADSORPTION CHARACTERISTICS[J];化工学报;2001-02
5 YU Xiao, ZHAO Rui *, FANG Mei, XIONG Shao Xiang, SU Tian Sheng, LIU Guo Quan [WT5”BX](Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China) [WT5HZ];Affinity Chromatography Based on the Oligohistines as the Ligands[J];高等学校化学学报;2000-04
6 Chai Hong ; Chen Huanlin; Liu Moe(Department of Biochemical Engineering .College of Chemical Engineering, Zhejiang University, Hangzhou 310027);Separation technology of immobilized metal ion affinity membrane(Ⅱ)Recent developments and application prospects[J];膜科学与技术;1999-05
7 Chai Hong,Chen Huanlin, Liu Moe (Department of Biochemical Engineering, College of Chemical Engineering, Zhejiang University, Hangzhou 310027);Separation technology of immobilized metal ion affinity membrane (Ⅰ) Method and theory[J];膜科学与技术;1999-04
8 ZHENG Yu, CHEN Huan Lin *, LI Jing, CHAI Hong, LIU Mo E [WT5”BX](College of Chemical Engineering, Zhejiang University, Hangzhou 310027, China) [WT5HZ];Studies on the Affinity Membrane for Adsorption and Purification of Lysozyme(Ⅰ) Influence of Low temperature Oxygen Plasma Treatment on Surface Structure and Adsorption Properties of the Affinity Membrane[J];高等学校化学学报;1999-08
9 LI Jing, CHEN Huan Lin *, CHAI Hong (College of Chemical Engineering, Zhejiang University, Hangzhou 310027, China);Studies on the Affinity Membranes for Adsorption and Purification of Lysozyme(Ⅱ) Adsorption Characteristics of Metal Chelating Affinity Membranes for Lysozyme[J];高等学校化学学报;1999-08
10 Zhou Dongmei\ Zou Hanfa\ Ni Jianyi\ Yang Li\ Jia Lingyun\ Zhang Yukui (National Chromatographic R & A Center,Dalian Institute of Chemical Physics,The Chinese Academy of Sciences,Dalian 116011);Fast Assay and Mini-preparation of Protein by High Performance Membrane Affinity Chromatography[J];生物工程学报;1998-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Sui Chunhong;Wang Cheng;Dong Shunfu;Han Liqin;Department of Pharmacy,Jilin Medical College;Department of Basic Medical Sciences,Jilin Medical College;;Immobilization of Glucoamylase onto the Electrospinning PEI/PVA Composite Nanofiber Membrane via Ionic Adsorption[J];生物技术通报;2015-02
2 SUN Qian;YAO Yao;CHENG Li;GU Xiao-yan;LIU Zheng-zhou;GU Jin-feng;Hash Water Quality Analysis Instrument ( Shanghai ) Co. ,Ltd.;Sinopharm Chemical Reagent Co. ,Ltd.;;Precast Reagent——The New Trend in the Development of Chemical Reagent[J];化学试剂;2014-05
3 CHEN Fang-you;LUO Yong-ming;WU Yang-ming;JIANG Chen-xing;ZHANG Xue-lian;Pharmacy School of Jiangxi University of Traditional Chinese Medicine;;Applications of Affinity Chromatography in Natural Medicine Research[J];中国实验方剂学杂志;2014-11
4 CHEN You-ning(Department of Chemistry,Xianyang Teacher's College,Shannxi Xianyang 712000,China);The Research Progress of Chromatographic Techniques in Protein Separation and Analysis[J];广州化工;2013-12
5 Li Buhai,Zhang Yonghong(Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Comission,College of Chemistry and Material Science,South-Central University for Nationalities,Wuhan 430074,China);Preparation of Corn Cellulose Based Adsorbents with Metal-Chelated Affinity and Their Adsorption Behaviour for BSA[J];中南民族大学学报(自然科学版);2012-04
6 Cao Ding1 Fu Zhifeng1 Li Congju2(1.College of Material Sciences and Engineering,Beijing University of Chemical Technology,Beijing 100029;2.College of Material Sciences and Engineering,Beijing Institute of Fashion,Beijing 100029);Overview of the electrospinning technology for filtration[J];化工新型材料;2011-08
7 ;The Research and Application of Lysozyme[J];科技传播;2011-13
8 DAI Youyuan1,ZHANG Jin2,(1.Beijing Tsinghua Research and Development Institute,Beijing 100084,China; 2.College of Science,Beijing University of Posts and Telecommunications,Beijing 100876,China);Analysis with fields/fluids and separation process intensification[J];膜科学与技术;2011-03
9 XIAO Yingjun ZHANG Yuzhong ZHANG Fengli LI Hong Key Lab of Hollow Fibre Membrane Materials & Membrane Process of Ministry of Education,Tianjin Polytechnic University,Tianjin 300160,China;ADSORPTION AND DESORPTION PROPERTIES OF RESIN FILLED EVAL HOLLOW-FIBER MEMBRANE ADSORBENTS[J];离子交换与吸附;2010-06
10 SUN Yanwei,WANG Bing(Key Laboratory of Hollow Fiber Membrane Material and Membrane Process, Ministry of Education,School of Environmental and Chemical Engineering,Tianjin Polytechnic University,Tianjin 300160,China);Preparationand performance research of a novel aminomethyl polysulphone affinity membrane[J];膜科学与技术;2010-05
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