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《Journal of Instrumental Analysis》 2001-01
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Determination of Plant Intrinsic Hormones by Reversed-phase High- performance Liquid Chromatography

XIE Jun, ZHANG Yi _ zheng (Molecular Biological laboratory, College of Life Science, Sichuan University, Chengdu 610064, China)  
A method for the determination of 5 intrinsic hormones: zeatin(ZT), gibberellin(GA3), kinetin(KT), 3 _ indoleacetic acid(IAA) and abscisic acid(ABA), in plant by reversed _ phase high _ performance liquid chromatography with μ Bondapak C18 column and UV detector was developed. The optimal chromatographic conditions were selected. An acetonitrile-methanol-0.6 % acetic acid system was used as mobile phase and 6 _ N _ benzyl adenine as an internal standard. A method for the extraction of the 5 hormones and the extract preparation was established. A sample of potato was analyzed. The mass fractions of ZT, GA3, KT, IAA and ABA are 0.298 5×10-9, 253.2×10-9, 2.154×10-9, 112.5×10-9 and 0.652 9×10-9, respectively. The proposed method is simple, convenient and sensitive.
【CateGory Index】: O657.7
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