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《Journal of Instrumental Analysis》 2008-02
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Selective Determination of Uric Acid in the Presence of Excessive Ascorbic Acid at Polymethylene Blue Modified Gold Electrode

MA Yan-rong,GAO Zuo-ning(Key Laboratory of Energy Sources and Chemical Engineering,College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China)  
The electrochemical properties of polymethylene blue(PMB)modified gold electrode(PMB/Au)were investigated by cyclic voltammetry and electrochemical impedance spectra(EIS),the electrochemical behaviors of ascorbic acid(AA) and uric acid(UA) at PMB/Au were also studied.The experimental results showed that the anodic peak potential of AA and UA shifted negatively and that the anodic peak current increased at PMB/Au electrode more than at bare gold electrode.The anodic peak of UA could be separated from AA with 430 mV at PMB/Au electrode by differential pulse voltammetry(DPV)and AA did not interfere the measurement of UA.The catalytic oxidation peak current was linearly dependent on the UA concentration.The linear range of the calibration curve was 5.0×10-6-8.0×10-3 mol·L-1 with a correlation coefficient of 0.998 7 in the presence of 1.0 mmol·L-1AA.The detection limit of UA was 1.0 μmol·L-1 in the presence of 500-fold AA.The method has been applied to the determination of UA concentration in human urine samples with satisfactory result.
【Fund】: 宁夏高等学校科学研究基金资助项目(200623)
【CateGory Index】: O657.1
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