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《Journal of Instrumental Analysis》 2010-10
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HPLC-MS Analysis and Activity Evaluation of Flavonoids of Apocynum venetum Leaves before and after Hydrolyzed by Enzyme

ZHANG Yu-chi1,LIU Chun-ming1,LIU Zhi-qiang2,WU Gui-mei1,LI Sai-nan1(1.The Central Laboratory,Changchun Normal University,Changchun 130032,China;2.Green Chemistry and Process Laboratory,Changchun Institute of Applied Chemistry,Chinese Academy of Science,Changchun 130022,China)  
The major flavonoids and its hydrolysates in Apocynum venetum leave were analyzed by high performance liquid chromatography coupled with electrospray ionization mass spectrometry(HPLC-ESI MS).The experimental results indicated that the flavonoids extracted from Apocynum venetum leave had the hydrophilic characteristic,which contained quercetin-3-O-β-D-galactosyl-β-D-glucopyranoside,rutin,kaempferol-3-O-β-D-rutinnoside,isoquercetin,avicularin and acetyled hyperoside.After hydrolyzed by complex enzyme of β-glucosidase and α-rhamnosidase,the compounds contained in the extract of raw plant materials of Apocynum venetum were transformed into hydrophobic compounds,e.g.hyperoside,isoquercetin,acetyled hyperoside,astragalin,trifolin,quercetin and kaempferol.The experimental results indicated that quercetin-3-O-β-D-galactosyl-β-D-glucopyranoside,kaempferol-3-O-β-D-rutinnoside and avicularin were the major compounds of Apocynum venetum before hydrolysis,hyperoside,isoquercetin,avicularin,quercetin and kaempferol were the major compounds in hydrolysates.Active evaluation experiment results also showed that the pc 12 cell proliferation of three dose groups after hydrolysis were 68.26%,101.69% and 105.05%,respectively,which raised 14.61%,22.48% and 20.22% respectively,compared with those of three dosage groups before hydrolysis.Therefore,the hydrolysis of β-glucosidase and α-rhamnosidase could be effectively converted flavone glycosides in Apocynum venetum leave into aglycones,and enhanced evidently the anti-depressant ability of flavone glycosides.
【Fund】: 国家自然科学基金资助项目(30970299);; 吉林省科技厅重点资助项目(20090936)
【CateGory Index】: R284
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