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《Journal of Instrumental Analysis》 2011-01
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Determination of Urine Protein by Sulfur Chlorophenol N-Cu(Ⅱ)-HSA-OP System

HU Qiu-luan1,FENG Ai-qing2,ZHAO Shu-zhen1,LIU Shang-cai3(1.College of Chemistry and Chemical Engineering,Luoyang Normal University,Luoyang 471022,China;2.Department of Life Science,Luoyang Normal University,Luoyang 471022,China;3.Luoyang Orthopedic Hospital,Luoyang 471002,China)  
In pH 3.0 Britton-Robinson buffer,sulfur chlorophenol N bonded with Cu(Ⅱ) to form a blue complex,and the complex reacted with protein to form a dark blue multi-complex at room temperature which presents a maximum absorption at 724 nm and a red shift of 174 nm comparing with that of sulfur chlorophenol N.A new method for the determination of proteins was established based on the reaction of multi-compound substance.The spectral characterisitics of the system and its influencing factors were investigated.Under the optimal conditions,the apparent molar absorptivity and linear range for HSA were 3.06×105 L·mol-1·cm-1 and 20-120 mg·L-1,respectively.With the presence of polyoxyethylene octyphenyl ether(OP),the sensitivity of the method could be increased by 31%.The mehtod was applied in the determination of urine protein with satisfactory results.And the sensitivity of the method is 16 times higher than that of the Biuret method.
【Fund】: 河南省科技厅科技攻关重点项目(102102110154);; 河南省教育厅自然科学基金资助项目(200510482009)
【CateGory Index】: R446.1;O657.32
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