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《Journal of Tianjin Normal University(Natural Science Edition)》 2018-02
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Effects of Edwardsiella tarda stimulation on the expression of differential gene in transcriptome of Paralichthys olivaceus

ZHOU Mi;ZHENG Jinhui;LI Qingya;GENG Xuyun;PAN Baoping;SUN Jinsheng;GAO Hong;College of Life Sciences,Tianjin Normal University;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University;Tianjin Aquaculture Disease Prevention & Treatment Center;  
To explore the response of gene expression of Paralichthys olivaceus to the Edwardsiella tarda stimulation,the transcriptome sequencing was conducted on the kidneys of stimuluted and unstimuleted P. olivaceus by E. tarda by Illumina HiSeq,and the differential genes were analyzed by bioinformatics,including GO(gene ontology),SNP(single nucleotide polymorphism)analysis,SSR(simple sequence repeats). The results showed that 222 980 transcripts were spliced by Trinity software with a total length of 200 234 420 bp,an average length of 898 bp and an N50 of 1 886 bp. After annotating the assembled genes for gene function,99 808 sequences were annotated. A total of 2 502 differentially expressed genes were screened,with 1 280 genes up-regulated and 1 222 down-regulated. The GOseq results showed that the differentially expressed genes were significantly enriched over 38 GO terms,including 6 cellular components,12 biological processes and 20 molecular functions. A total of 277 KEGG pathways were enriched and 627 differential genes were significantly enriched to 20 signal pathways,among which eight were related to the signaling pathway,three were related to development and nine were related to some diseases.
【Fund】: 天津市自然科学基金重点资助项目(14JCZDJC34200)
【CateGory Index】: S943
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