Nigral Cells Transplanted into the Striatum of PD-RatS: Effect of NGF on Grafted Cells
Liu Rengang, Liu Yunhua, Zhu Changgeng Department of Anatomy, Faculty of Basic Medical Sciences
Fatal nigral cell suspensions with or without nerve growth factor (NGF) were transplanted into the striatum of Parkinson disease(PD) rats with unilateral 6-OHDA induced lesions of the nigrostriatal pathway. Each two weeks after implantation, animals were tested for rotation behavior. 8 or 16 weeks after implantation, animals were perfused and prepared for Faglu-Sucrose fluorescence histochemistry, tyrosine hydroxylase(TH) immunocytochemistry and electron microscopy. The results show that apomorphine-induced rotation behavior shows a marked improvement in both groups and that the fibres ofgrafted cells treated with NGF are more numerous and longer than those untreated with NGF. The ultrastructures reveal that the TH-immunoreactive axons make synaptic contact with dendrites of host cells in the striatum of animals treated with NGF.