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《Acta Medicinae Universitatis Scientiae et Technologiae Huazhong》 2006-03
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Sensitivities of Cholangiocarcinoma Cells Transfected by Exogenous p27~(kip1) Gene to Chemotherapy

Luo Jian,Cao Zhihua~#,Liu Minfeng et alDepartment of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030  
Objective To investigate the effects of exogenous p27~(kip1) gene on chemosensitivity of human cholangiocarcinoma cell line.Methods The recombinant vector was constructed and the mutated p27~(kip1) gene was transfected into human cholangiocarcinoma cell line(QBC_(939)).RT-PCR and Western blot were used to determine the expression of target genes.The effects of 5-fluorouracil(5-FU),mitomycin(MMC) and cyclophosphamide (CTX) on the transfected cells were detected by assaying the rate of apoptosis and growth inhibition by methabenzthiazuron(MTT) assay and flow cytometry(FCM). Results The exogenous p27~(kip1)gene was expressed effectively in the cells,and the expression enhanced the apoptosis and growth inhibition of QBC_(939) induced by 5-FU,MMC and CTX.The ratio of growth inhibition was increased significantly from 41.89 %(5-FU),45.59 %(MMC) and 38.91 %(CTX) to 56.15 %(5-FU),55.65 %(MMC) and 51.69 %(CTX) and apoptosis index from 13.76 %(5-FU),11.76 %(MMC),10.46 %(CTX) to 41.39 %(5-FU),35.94 %(MMC),34.46 %(CTX) respectively.Conclusion The exogenous p27~(kip1) gene transfer can remarkably increase the drug sensibility of the cholangiocarcinoma cells.The combination of p27~(kip1) gene therapy with chemotherapy may be more effective in cancer treatment.
【Key Words】： gene therapy adenovirus chemosensitivity cholangiocarcinoma
【Fund】： 国家“863”高技术研究发展计划资助项目(No.2002AA214061)
【CateGory Index】： R735.8
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