Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Medicinae Universitatis Scientiae et Technologiae Huazhong》 2008-01
Add to Favorite Get Latest Update

Effects of Adipose Stromal Cells on Allo-genetic Dendritic Cells and Its Possible Role

Wang Hongxiang,Zhao Shi,Mao Hong et al Department of Hematology,Wuhan Central Hospital,Wuhan 430014  
Objective To observe the effects of adipose stromal cells(ASC) on the phenotype and secreting cytokines of allogenetic dendritic cells(DC),and elucidate the possible role of the immunomodulation of ASC and the possible mechanism.Methods The supernatant of ASC and ASC themselves were co-cultured with allo-genetic DC.Flow cytometry was used to detect the expression of CD80,CD83 and CD86.Enzyme-linked immunoabsorbent assay was used to detect the secretion level of IL-12,and RT-PCR to detect the expression level of indoleamine 2,3-dioxyenase(IDO).Results The expression level of CD80,CD83 and CD86 on the surface of DC was reduced by(16.9±5.1)%,(8.4±3.7)% and(25.9±6.5)%,respectively after the supernatants of ASC were added in the culture system of DC.The secretion of IL-12 was decreased by(168.3±51.2) pg/ml and(180.5±56.7) pg/ml,while the expression of IDO was up-regulated in the presence of ASC supernatant and ASC respectively.There were significant differences between the experimental group and control group(P0.05).Conclusion Via cell-to-cell contact and secretion of some kinds of cytokines ASC can affect the function of DC,negatively modulate the immunity and induce the immune tolerance.
【Fund】: 湖北省科技攻关计划资助项目(No2005AA304B03)
【CateGory Index】: R329;R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Yong reviewing,ZHAO Jian-ning checking(Department of Orthopedics,Jinling Hospital,Nanjing 210002,Jiangsu,China);Progress in researches of adipose-derived stem cells[J];Journal of Medical Postgraduates;2009-10
【Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Bin-gong~*,ZENG Qiu-tang,WANG Hong-xiang,et al.~*Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Comparison of mesenchymal stem cells from adipose tissue and bone marrow[J];Chinese Journal of Organ Transplantation;2006-03
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 WU Lei,CHEN Ruo-Kun,YANG Liang, YANG Zhuan-Yi,CHAI Zhi-Yong, FANG Jia-ShengDepartment of Neurosurgery, Xiangya Hospital, Central South University, Changsha 410008, China;Differentiation of adipose-derived stem cells into nerve stem cells across embryonic layer[J];Journal of the Fourth Military Medical University;2009-01
2 Chi-Dan Wan, Rui Cheng, Hong-Bo Wang and Tao LiuDepartment of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Immunomodulatory effects of mesenchymal stem cells derived from adipose tissues in a rat orthotopic liver transplantation model[J];国际肝胆胰疾病杂志(英文版);2008-01
3 Wang Fu-ke1,Liu Liu1,Zhao De-ping2,Dai Xiao-ming1,Li Yi-song1,Li Zong-fang1 1Department of Plastic Surgery,2Experimental Center,First Affiliated Hospital,Kunming Medical College,Kunming 650032,Yunnan Province,China;Osteogenic property of rat bone marrow mesenchymal stem cells versus adipose mesenchymal stem cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-47
4 Chen Wei,Jiang YiTianjin Third Central Hospital,Tianjin 300170,China;Reparation of tibial defects using adipose-derived stromal cells combined with bovine decalcified bone in rabbits[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-34
5 Jiang Yi,Liu Zhi,Han Li-qiang,Zhu Zheng-yan,Tian Yong-gangDepartment of Orthopaedics,Tianjin Third Central Hospital,Tianjin 300170,China;Osteogenic activity of in vitro cultured human adipose stromal cells versus bone marrow stromal cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-23
6 Wang Fu-ke1,Liu Liu1,He Xiao-guang1,Zhao De-ping2,Dai Xiao-ming1,Li Yi-song1 1Department of Head and Neck Surgery,2Experimental Center,First Affiliated Hospital,Kunming Medical College,Kunming 650032,Yunnan Province,China;Ossification induction and phenotype of a special cell in culture process of rat adipose-derived stem cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2010-40
7 Yang Bo,Liang Jing-qun,Liu Tao,Wang Wang,Zhang Xiang PLA Institute of Physical Education,Guangzhou 500052,Guangdong Province,China;Stem cells involved in the tissue repair of sports injury[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2011-10
8 Lu Tan, Li Xiao-wei, Zhang Jiang-feng, Xu Hai-bin Department of Orthopedic Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China;Poly-L-lysine bio-effects on the three-dimensional culture of rat adipose-derived stem cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2011-40
9 Liu Xiao-tan 1 , Xu Hai-bin 2 , Lu Tan 2 1 Department of Orthopedics, the Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan Province, China 2 Department of Orthopedics, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, Henan Province, China;Adipose-derived stem cells differentiate into osteoblasts and chondrocytes[J];Chinese Journal of Tissue Engineering Research;2013-14
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 DU Yi-ri,FU Xiao-bing,LI Cun-bao,et al. (Key Laboratory of Wound Repair,304 Medical Department,The General Hospital,PLA,Beijing 100037,China);In vivo studies on the differentiation of mesenchymal stem cells to skin tissues induced by epidermal growth factor[J];Journal of Traumatic Surgery;2005-02
2 NI Yun-Feng1, LI Xiao-Fei1, LIU Yuan2, LEI Zhan-Jun2, LU Qiang11Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China, 2Department of Pathology, Stomatological Hospital, Fourth Military Medical University, Xi'an 710033, China;Experimental study on chondrogenesis by co-culture of rabbit mesenchymal stem cells and chondrocytes[J];Journal of the Fourth Military Medical University;2006-07
3 YANG Ya-Jun,ZHU Qing-Sheng Institute of Orthopedics,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China;Differentiation of rat adipose-derived stem cells into chondrogenic phenotype with induction of cartilage-derived morphogenetic protein (CDMP1) growth factor in vitro[J];Journal of the Fourth Military Medical University;2008-04
4 HAO Peng1,LI Sheng-fu2,PEI Fu-xing 1 ,et al.(1.Department of Orthopedic Surgery,2.Lab of Transplant Engineering and Immunology, West China Hospital,Sichuan University,Chengdu Sichuan 610041,China);Isolation of Chondrocytes from the Adult Rabbit Model of Impacting Injury of Articular Cartilage and Observation of Their Biological Characteristics during Cultivation in Vitro[J];West China Medical Journal;2007-01
5 WANG Bei-yue reviewing,ZHAO Jian-ning checking (Department of Orthopaedics,Nanjing School of Clinical Medicine,Southern Medical University/ Nanjing General Hospial of Nanjing Military Command,PLA,Nanjing 210002,Jiangsu,China);Tissue engineering scaffolds in spinal cord injury[J];Journal of Medical Postgraduates;2008-05
6 WANG Jin-liang reviewing, ZHAO Jian-ning checking (Department of Orthopedics, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing 210002, Jiangsu, China);Update of composite scaffold materials in cartilage tissue engineering[J];Journal of Medical Postgraduates;2009-02
7 ZHANG Yong reviewing,ZHAO Jian-ning checking(Department of Orthopedics,Jinling Hospital,Nanjing 210002,Jiangsu,China);Progress in researches of adipose-derived stem cells[J];Journal of Medical Postgraduates;2009-10
8 TAN Tao,QIN Shujian,WANG Chao,REN Guangda,PEI Dan(Department of Anatomy,Liaoning Medical University,Jinzhou 121001 China);Influence of Age on Osteogenic Differentiation of Rat Adipose-derived Stem Cells[J];Journal of Liaoning Medical University;2009-01
9 LU Wei,JIN Yan,ZHANG Yong-Jie.Department of Oral Histopathology,College of Stomatology,Fourth Military Medical University,Xi'an 710032,China;A study of biological characteristics of adipose tissue-derived stem cells(ADSCs) from SD rat in vitro[J];Journal of Clinical Stomatology;2006-07
10 LI Chun-Ming,LIU Yi(Department of Burns and Plastic Surgery,Lanzhou General Hospital,Lanzhou Command,CPLA.730050,Gansu,China);The biological characteristics of mesenchymal stem cells from human adipose tissue[J];Chinese Journal of Aesthetic Medicine;2007-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved