The experimental study of the impacts of the magnetic field on proliferation activity in rat pancreatic islet cells

ZHANG Hao-nan;WANG Yi-min;YANG Lin;WANG Yun-hua;MENG Qing-nan;MENG Yan-ni;School of Chinese Medicine Engineering,Tianjin University of TCM;  

[Objective] To explore the impact of magnetic field of different intensities on the proliferation and activity in rat islet cells under the normal and hypoxic culture conditions. [Methods] According to the islet cells growth curve case, we selected cells cultured3 days for the experiment. Under the normal and hypoxic culture conditions, using 3.4,5.6,13.8,27.8,52.6,107.0,178.9 mT magnetic field intervene to magnetic A-G group. Control cells received no magnetic field exposure. We used MTT assay to detect cells activity for each group after 72 hours. Using 44.8,90.6,182.1 mT magnetic field intervene to magnetic a-c group. Control cells received no magnetic field exposure. Using Flow Cytometry detect cells proliferation for each group after 72 hours. [Results] The cultured cells grew linearly with optical density(OD) of(0.067±0.021) after 2 days and(0.449±0.113) after 5 days. A-G groups of MTT compared with the control group under the normal culture conditions, and it was no statistically significant(P0.05). Under the hypoxic culture conditions, magnetic MTT values higher in group A(P0.01). Under the normal culture conditions, cells proliferation increased as the magnetic field strength decreasing first and then increasing in group a-c. And under the hypoxic culture conditions, cells proliferation increased with magnetic field strength. [Conclusion] Under the normal culture conditions, it has no effect on the activity of rat pancreatic islet cells under different intensity permanent magnetic field and it can improve islet cells proliferation on high-intensity magnetic field. Under the hypoxic culture conditions, low-intensity magnetic field can increase the activity of rat pancreatic islet cells, high-intensity magnetic field can increase islet cells proliferation.

【Key Words】： permanent magnet magnetic field islet cell MTT Flow Cytometry
【Fund】： 国家自然科学基金项目(30873467)
【CateGory Index】： R329

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