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《Natural Product Research and Development》 2003-06
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EFFECTS OF PUERARIN ON RENAL FUNCTION AND EXPRESSIONS OF MMP-3 AND TIMP-1 IN THE KIDNEY OF DIABETIC RATS

LIU Shu-xia1,DUAN Hui-jun 1,ZHANG Yu-jun1,CHEN Zhi-qiang2 HE Ning1,LIU Qing-juan1,LI Ying-min1 (1.Department of Pathology,Hebei Medical University,Shijiazhuang 050017,China; 2.The Institute of Combined Traditional Chinese and Western Medicine,Hebei Medical University,Shijiazhuang 050017,China)  
Objective:To investigate the effects of puerarin on renal structure?function and expressions of MMP-3 and TIMP-1 in the kidney of diabetic rats.Methods:Uninephrectomized STZ-induced male wister rats were used.Puerarin was delivered daily by the intraperitoneal injection from the third day of induction to diabetes for 16 weeks.Using in situ hybridization and immunohistochemistry to detect TIMP-1mRNA expression and MMP-3?TIMP-1?collagen Ⅳ and Laminin expressions in diabetic kidneys with image analysis system;Flow cytometry was used to detect the expressions of MMP-3?TIMP-1.Results:BUN?Ccr and Upro in diabetic rats were significant higher than that in control group.Compared with those in kidneys of control group,diabetic kidneys showed increased expressions of both MMP-3?TIMP-1 proteins and excessive deposition of glomeruli extracellular matrix(P0.05).The blood glucose and the level of expressions of MMP-3?TIMP-1?collagen Ⅳ and Laminin were reduced by puerarin treatment,renal function was improved.Conclusions:Puerarin has some renal protective effect on diabetic nephropathy partly through inhibition of excessive deposition of glomeruli extracellular matrix by down-regulating MMP-3 and TIMP-1 expressions besides reducing the blood glucose.
【Fund】: 教育部归国博士基金资助项目J980 60 1
【CateGory Index】: R285.5
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