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《Natural Product Research and Development》 2005-04
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Extraction of Saponins in Panax notoginseng with Conbined Enzymolysis

LI Yuan-bo~*,YIN Hui-an,TANG Ming-lin,AN Lian-ying,ZHOU Lin (Department of Chemical Engineering and Pharmaceutics,Chengdu University of Technology,Chengdu 610059,China)  
We took total content of saponins and extraction rate as index to study the advantages and disadvantages of five extraction method,including reflux,percolation,cellulase enzymolysis,pectinase enzymolysis and conbined enzymolysis.In order to optimize the extraction procedure, we adopted single-factor method and orthogonal experiment design(4 factors and 3 levels)to determine the extraction condition.The optimal extract conditions are as fo1lows:15 U Cellulase anzyme and 140 U pectinase enzyme for 1 g Panax notoginseng,enzymolysis temperature 50 ℃,enzymic treatment 2.5 h at 4.5 pH in 80% alcohol.The content of total saponins in the extraction solution is 12.01%,and extraction rate is 35.82% under these conditions.
【CateGory Index】: R284
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