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Physicochemical Properties and Antitumor Activity of Polysaccharide DCPP1a-1 from Suspension-cultured Protocorms of Dendrobium candidum

HE Tie-guang1,2,3,YANG Li-tao1,LI Yang-rui3,WAN Can-qin2,HUANG Dao-ping41College of Agronomy,Guangxi University,Nanning 530005,China;2Institute of Biotechnology,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;3Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China;4Guangxi Research Centre of Analysis,Nanning 530022,China  
The polysaccharide DCPP1a-1 was isolated from protocorms of Dendrobium candidum through water extraction,deprotein,ethanol precipitation,and DEAE-cellulose column chromatography respectively,and was further purified by Sephadex G-200 column chromatography.Its structure features were analyzed by periodate oxidation,TLC,HPLC,UV and IR spectra.Its antitumor activity in vivo was studied.The results showed that DCPP1a-1 was a single homogeneous component,and it had α-pyranoside with(1→6,1→2 or 1→4,1→3)glycosidic linkages,where 1→6,1→2 or 1→4 and 1→3 took up 4.0%,52.1% and 44.9%,respectively.The molecular weight of DCPP1a-1 was estimated to be 189 kD.Chemical component analysis showed that it was composed of mannose and glucose in a molar ratio of 7.015∶1,respectively.As a result,it was a novel polysaccharide and was first obtained from suspension-cultured protocormns of Dendrobium candidum.DCPP1a-1 had different anti-tumor effects on tumor H22-bearing mice at dose of 50,150 and 250 mg/kg and the inhibitory rates of three groups of DCPP1a-1 on tumor H22 were 28.6%,19.3% and 15.7%,respectively.Especially the inhibitory rate to tumor H22 was the highest at low dose(P0.05).It could also significantly raise thymus and spleen index at low dose(P0.05).
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