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《Natural Product Research and Development》 2007-04
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Physicochemical Properties and Antitumor Activity of Polysaccharide DCPP1a-1 from Suspension-cultured Protocorms of Dendrobium candidum

HE Tie-guang1,2,3,YANG Li-tao1,LI Yang-rui3,WAN Can-qin2,HUANG Dao-ping41College of Agronomy,Guangxi University,Nanning 530005,China;2Institute of Biotechnology,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;3Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China;4Guangxi Research Centre of Analysis,Nanning 530022,China  
The polysaccharide DCPP1a-1 was isolated from protocorms of Dendrobium candidum through water extraction,deprotein,ethanol precipitation,and DEAE-cellulose column chromatography respectively,and was further purified by Sephadex G-200 column chromatography.Its structure features were analyzed by periodate oxidation,TLC,HPLC,UV and IR spectra.Its antitumor activity in vivo was studied.The results showed that DCPP1a-1 was a single homogeneous component,and it had α-pyranoside with(1→6,1→2 or 1→4,1→3)glycosidic linkages,where 1→6,1→2 or 1→4 and 1→3 took up 4.0%,52.1% and 44.9%,respectively.The molecular weight of DCPP1a-1 was estimated to be 189 kD.Chemical component analysis showed that it was composed of mannose and glucose in a molar ratio of 7.015∶1,respectively.As a result,it was a novel polysaccharide and was first obtained from suspension-cultured protocormns of Dendrobium candidum.DCPP1a-1 had different anti-tumor effects on tumor H22-bearing mice at dose of 50,150 and 250 mg/kg and the inhibitory rates of three groups of DCPP1a-1 on tumor H22 were 28.6%,19.3% and 15.7%,respectively.Especially the inhibitory rate to tumor H22 was the highest at low dose(P0.05).It could also significantly raise thymus and spleen index at low dose(P0.05).
【Fund】: 广西农业科学院科技发展基金项目(2004012)
【CateGory Index】: R284
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【References】
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1 LUO Ao-xue et al(Dujiangyan Campus of Sichuan Agricultural University,Dujiangyan,Sichuan 611830);Study on the Protein Removal of the Crude Polysaccharide from Dendrobium[J];Journal of Anhui Agricultural Sciences;2008-29
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Wei1,ZHU Jin-hua1,WANG Min2,LI Chao-jun2, ZHANG Cheng-wu 2,3 (1.Jiangsu Staff Medical College,Nanjing 210029,China; 2.Molecular & Medical Biotechnology Laboratory,Nanjing Normal University, Nanjing 210097,China; 3.School of Biomedical Science,Curtin University of Technology,Australia);ISOLATION AND PURIFICATION OF INTRACELLULAR POLYSACCHARIDE FROM SPIRULINA MAXIMA AND ITS PRIMARY STRUCTURE[J];Natural Product Research and Development;2004-06
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 HE Tie-guang1,2,3,YANG Li-tao1,LI Yang-rui3,WANG Can-qin1,HUANG Dao-ping4(1.Institute of Biotechnology,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;2.College of Agronomy,Guangxi University,Nanning 530005,China;3.Guangxi Crop Genetic Improvement and Biotechnology Lab,Nanning 530007,China;4.Guangxi Research Centre of Analysis,Nanning 530022,China);Isolation,Purification and Preliminary Structure Analysis of Polysaccharide DCPP3c-1 in Suspension-cultured Protocormns of Dendrobium Candidum[J];Journal of Instrumental Analysis;2008-02
2 SUN Xiao-xue1*,SUN Wei-dong1,SHI De-fang2,ZHANG Ye-hui11Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;2Hubei Academy of Agriculture Sciences,Wuhan 430064,China;Protein Removal from Polysaccharide Extract of Cactus[J];Natural Product Research and Development;2007-01
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【Secondary References】
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1 WEI Ming et al (Department of Biochemical Engineering,Anhui College of Engineering Science and Technology,Wuhu,Anhui 241000);Kinetics Study on the Proliferation of Protocorm-like Body of Dendrobium huoshanense in Phosphorus-limiting Culture and Its Polysaccharide Synthesis[J];Journal of Anhui Agricultural Sciences;2009-24
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