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《Natural Product Research and Development》 2010-01
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Technics of Melanin Extraction from Ink of Sepia esculenta Hoyle by Enzymatic Method

LI Xiao,LI He-sheng Faculty of Life Science and Biotechnology of Ningbo University,Zhejiang Ningbo,315211,China  
The Sepia esculenta Hoyle was used as raw material in this experiment to extract melanin.Comparing the effects of melanin extraction by different proteinases,alcalase was selected from trypsin,papain,pepsin and neutral protease.Using single-factor test,the effects of different factors on extraction of melanin were investigated.With orthogonal test,the optimal parameters of extraction of Sepia esculenta Hoyle melanin were as follows:the 4200 U/g alcalase was added for the hydrolisys for 8 h in the condition of 50 ℃,pH 7.4 and substrate concentration 2%.
【Fund】: 浙江省重大科技专项和优先主题计划项目(2009C03017-3)
【CateGory Index】: TS254.9
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