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《Natural Product Research and Development》 2015-04
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Optimization of Extraction Process of Total Ginsenoside from Adventitious Roots of Panax ginseng C. A. Meyer by Response Surface Methodology

ZHANG Ru;ZHANG Bian-ling;XIE Tao;LI Gu-cai;LUO Zhi-yong;College of Chemistry and Chemical Engineering,Hunan Institute of Engineering;Molecular Biology Research Center,School of Life Sciences;  
In this study,total ginsenoside was extracted from the adventitious roots of Panax ginseng C. A. Meyer by ethanol extraction method. The extraction process was optimized by response surface methodology(RSM). Using the total yields of Rb1,Rb2,Rc,Rd,Re and Rg1 as responses,main factors,including extraction temperature,ethanol concentration and liquid-to-solid ratio,were investigated. Based on the results of single factor test,response surface methodology with 3 factors and 3 levels was applied according to Box-Behnken experimental design principle. The relationships between the yield of total ginsenoside and factors were investigated by response surface experiments,and then the regression equations were established. The optimal results were simulated using the regression models based on the value of response surface. The optimized extraction process of total ginsenoside were determined as follows:extraction temperature of 70 ℃,ethanol concentration of 73. 50% and solid-liquid ratio of 1∶ 34. 50(g/m L),under which the yield of total ginsenoside was up to 1. 86 ± 0. 01%.
【Fund】: 湖南省自然科学基金(11JJ6009);; 湖南省教育厅(11C0329);; 国家级大学生创新创业训练计划(201411342004);; 湖南省大学生研究性学习和创新性实验计划(2014-248)
【CateGory Index】: R284.2
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