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《Natural Product Research and Development》 2015-04
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Optimization of Extraction Process of Total Ginsenoside from Adventitious Roots of Panax ginseng C. A. Meyer by Response Surface Methodology

ZHANG Ru;ZHANG Bian-ling;XIE Tao;LI Gu-cai;LUO Zhi-yong;College of Chemistry and Chemical Engineering,Hunan Institute of Engineering;Molecular Biology Research Center,School of Life Sciences;  
In this study,total ginsenoside was extracted from the adventitious roots of Panax ginseng C. A. Meyer by ethanol extraction method. The extraction process was optimized by response surface methodology(RSM). Using the total yields of Rb1,Rb2,Rc,Rd,Re and Rg1 as responses,main factors,including extraction temperature,ethanol concentration and liquid-to-solid ratio,were investigated. Based on the results of single factor test,response surface methodology with 3 factors and 3 levels was applied according to Box-Behnken experimental design principle. The relationships between the yield of total ginsenoside and factors were investigated by response surface experiments,and then the regression equations were established. The optimal results were simulated using the regression models based on the value of response surface. The optimized extraction process of total ginsenoside were determined as follows:extraction temperature of 70 ℃,ethanol concentration of 73. 50% and solid-liquid ratio of 1∶ 34. 50(g/m L),under which the yield of total ginsenoside was up to 1. 86 ± 0. 01%.
【Fund】: 湖南省自然科学基金(11JJ6009);; 湖南省教育厅(11C0329);; 国家级大学生创新创业训练计划(201411342004);; 湖南省大学生研究性学习和创新性实验计划(2014-248)
【CateGory Index】: R284.2
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LIU Yong-ling;WANG Ping;CHEN Tao;CHEN Chen;LI Yu-lin;Northwest Institute of Plateau Biology,CAS;Graduate University of Chinese Academy of Sciences;;Optimization of Ultrasonic-assisted Extraction of An Imidazole Alkaloid from Aconitum pendulum Busch by Response Surface Methodology[J];Natural Product Research and Development;2014-08
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 CUI Xue-zheng et al(College of Life Sciences,Jilin Agricultural University,Changchun,Jilin 130118);Research Advances in Panax ginseng Genetic Engineering[J];Journal of Anhui Agricultural Sciences;2012-13
2 NIU Xiao-xue,CUI Xu-sheng,SU He,GUO Yu-hai,DONG Xue-hui(College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China);Influences of micronutrient fertilizer and methyl jasmonate treatments on the content of compounds in Lonicera japonica[J];China Journal of Traditional Chinese Medicine and Pharmacy;2013-02
3 PENG Fei,ZHONG Xiang-yun,TAN Zhao-yang,LEI Si-qi,LI Yun-yao(TCM University of Hunan,Changsha,Hunan 410208,China);Study of Lycoris aurea on induction and culture of adventitious root in vitro[J];Journal of Traditional Chinese Medicine University of Hunan;2008-01
4 XU Li-xin,ZHAO Shou-jing,LIANG Yan-long,QIAN Yan-chun,JIN Shi-kun(College of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China);Effect of extrinsic regulators on growth and ginsenoside content of hairy roots of Panax ginseng[J];Journal of Jilin University(Engineering and Technology Edition);2010-06
5 Hosakatte Niranjana Murthy1, 2, Eun Joo Hahn1, and Kee Yoeup Paek1 1 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju 361-763, South Korea 2 Department of Botany, Karnatak University, Dharwad 580003, India;Adventitious Roots and Secondary Metabolism[J];Chinese Journal of Biotechnology;2008-05
6 HOU Chun-xi ZHAO Shou-jing LIANG Yan-long WANG Jian-hua(College of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China);Advances in Genetic Transformation of Panax Ginseng[J];China Biotechnology;2009-09
7 ZHAN Qing-Qing1a,1b,2,JIN Yue1a,WEI Jian-He1a,ZHANG Jie2,SUI Chun1a 1.a.Institute of Medicinal Plant Development,Beijing 100193;b.Hainan Branch Institute of Medicinal Plant,Wanning 571533;Chinese Academy of Medical Sciences & Peking Union Medical College;2.College of Life Sciences,Northeast Forestry University,Herbin 150040;China;Cultivation of Adventitious Roots and Effect of Methyl Jasmonate on its Saikosaponins Contents for Bupleurum chinense DC.[J];Letters in Biotechnology;2011-01
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 WU Lin1, 2, ZHANG Fan1, 2, 3, Halmurat.UPUR1, 3, ZHAO Ting-ting2(1Xinjiang Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011, China; 2Chinese Medicine College, Xinjiang Medical University, Urumqi 830011, China; 3Pharmaceutical Post-doctoral Station, Xinjiang Medical University, Urumqi 830011, China);Influences on contents of alkaloid in Aconitum pendulum Busch samples processed by different methods[J];China Journal of Traditional Chinese Medicine and Pharmacy;2011-06
2 Zou Jie,Wang Xuan(Yinchuan Energy Institute,Yinchuan 750105,China);The Research on Extraction Technology of Alkaloids[J];Guangdong Chemical Industry;2012-11
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