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《Natural Product Research and Development》 2015-10
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Optimization of Ultrasonic-assisted extraction of Total Flavonoids from Prunus divaricata Ldb Leave Using Response Surface Methodology

LIU Wei;HE Xiao-yan;CHEN Wen-qiang;ZHU Yan;DAI Si-yue;OUYANG Yan;University and College Key Lab of Natural Product Chemistry and Application in Xinjiang,Yili Normal University;  
The optimal conditions for the ultrasonic-assisted extraction of total flavonoids from Prunus divaricata Ldb leave were determined using response surface methodology. Based on single factor experiments,the influence of ethanol concentration,extraction temperature and solid-liquid ratio on the extraction yield of total flavonoids were investigated.The optimal conditions were determined as follows: ethanol concentration was 51%,extraction temperature was 72 ℃,solid-liquid ratio was 1∶ 45( g / m L),ultrasonic power was 300 W,for 2 times,each time 30 min,the yield of total flavonoids was 38. 27 mg / g,which accounts for 98. 3% of the theoretically predicted value. Compared with the traditional Soxhlet extraction,the ultrasonic-assisted extraction was more convenient,less time-consuming,higher extraction efficiency. Hence,the ultrasonic-assisted extraction can be used as an effective method for the extraction of total flavonoids from P. divaricata leave.
【Fund】: 新疆维吾尔自治区高校科研计划(XJEDU 2014S061);; 自治区教育厅普通高校重点实验室资助项目(2013YS HXZD03)
【CateGory Index】: R284.2
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