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《Chinese Journal of Soil Science》 2011-01
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Measurement of Catalase Activity in Soil by Ultraviolet Spectrophotometry

YANG Lan-fang1,ZENG Qiao1,2,LI Hai-bo1,YAN Jing-jing1(1.School of Resource and Environmental Science,Hubei University,Wuhan 430062,China;2.Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Lanzhou 730000,China.)  
Catalase is an important enzyme in soil.In order to find a simple,reliable and applicable method to measure the activity of soil catalase,the experiment to determine the catalase activity in soil by ultraviolet spectrophotometry was conducted.The results showed that the reproducibility of ultraviolet spectrophotometry was better than potassium permanganate volumetric method.The absorbency of solution of soil extract in 240 nm had no visible changes after stored 11 h.The results from 4 soils indicated that there was no significant difference between ultraviolet spectrophotometry and potassium permanganate volumetric method.The addition of sulfate acid before filtration in traditional volumetric method led to a colored and turbid solution,and long filtration time.The color and turbidity in soil extract was disadvantage to measure the absorbency.Adding alum saturated solution before filtration and then filtrating in a utensil with 5 ml sulfate acid(1.5 mol L-1) could gain a colorless and transparent soil extract and shorten the filtrating time.In conclusion,with high reproducibility and stability,simple operation,no special reagent and avoidance of the error in titrimetry,ultraviolet spectrophotometry was a valuable method and worth of popularization in determination of soil catalase activity.
【Fund】: 生物资源保护与利用湖北省重点试验(湖北民族学院)开放基金项目;; 湖北省教育厅重点项目(D20081010)
【CateGory Index】: S154.2
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