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《Acta Pedologica Sinica》 1993-04
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Chen Meici, Min Hang and Qian Zeshu (Zhejiang Agricultural University, 310029)  
With improved Hungate anaerobic technique, the amounts of methane——producing bacreria (MPB) in the submerged paddy soil planting rice crop were determined by the MPN (The Most Probable Number) method and the roll tube method at the same time. The esults obtained showed that the amounts of MPB during different growing periods of rice were significantly different, the amount of MPB grown on mixed substrate was the highest at the late tillering stage of early rice, up to 3.6×10~(10)/g dry soil; that on H_2/CO_2 increased significantly at the vigorously tillering stage of late rice, reaching 3.1×10~(11)/g dry soil at milk ripening stage; the amounts of MPB grown on formate and acetate were higher, about 3.7×10~8/g dry soil and 1.2×10~8/g dry soil respectively at the vigorously tillering stage of late rice; and that of MPB grown on methanol had no significant difference during the different growing periods being, about 3.5×10~5/g dry soil.The amounts of MPB had no marked difference in soil at different depths.A certain difference was found in the number of MPB under conditions of different kinds of fertilizers applied, and addtion of organic manure stimulated methane prodection.The dominant species of methanogenic bacteria in the paddy soil were Methanobacterium formicicum, Methanosarcina barkeri and Methanosarcina mazei.
【Fund】: 浙江省自然科基金
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