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《Acta Pedologica Sinica》 2004-03
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RAPID EXTRACTION AND PURIFICATION OF DNA IN FARMLAND SOILS CONTAMINATED WITH MIXED HEAVY METALS FOR PCR-DGGE ANALYSIS

Teng Ying Luo Yongming  Zhao Xiangwei Li Zhengao Song Jing Wu Longhua (State Key Laboratory of Soil and Agricultural Sustainable Development (Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China) (Soil and Environment Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China)  
Total community DNA was extracted and purified from soils contaminated with different levels of mixed heavy metals by FastPrep○R system, and then amplified by using eubacterial 16S rDNAs primers. Their PCR products were analysed by DGGE to obtain bacterial community patterns. The results showed that the FastPrep○R120 instrument with the FastDNA SPIN Kit for soil can be used to rapidly, effectively extract and purify DNA from polluted soils. The electrophoresis lanes obtained by PCR-DGGE were legible and distinguishable. The profiles of PCR-DGGE indicated that heavy metals accumulation at different levels markedly caused damage to farmland soils DNA, affecting the soil microbial richness and structure of dominating bacterial populations in the farmland ecological systems, and changing the structural diversity of microbial community. The FastPrep○R system is also suitable for rapid efficient extraction and purification of soil total community DNA from farmland soils polluted with mixed heavy metals and in turn the DNA can be used directly for PCR-DGGE analysis.
【Fund】: 国家杰出青年基金项目 ( 4 0 12 5 0 0 5 );; 国家重点基础研究发展规划项目 ( 2 0 0 2CB410 810、2 0 0 2CB410 80 9);; 中国科学院知识创新工程重要方向项目 (KZCX3 SW 42 9)资助
【CateGory Index】: X53
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【References】
Chinese Journal Full-text Database 10 Hits
1 LI Hui-min (ShangLuo Professional Technical College,Shaanxi ShangLuo 726000);Application of PCR Technique in Environmental Monitoring[J];Journal of Shangluo Teachers College;2005-04
2 WANG Shu-ying,MA Xiao-hua(Chemistry Department,Shangqiu Teachers College,Shangqiu 476000,China);Harm of the Heavy Metals Pollution in Soil and Its Remedies[J];Journal of Shangqiu Teachers College;2005-05
3 CHEN Cheng-Li, LIAO Min~*, ZENG Lu-Sheng (MOE Key Laboratory,Environmental Remediation and Ecosystem Health, College of Environmental and Resources Sciences, Zhejiang University, Hangzhou, 310029,China).;Methods to measure the microbial community structure and functional diversity in polluted soils[J];Acta Ecologica Sinica;2006-10
4 SHAN Guobin 1,2 ,JIN Wenbiao3,LIN Jikan 1,4 ,XING Xinhui2 ( 1Baolimei Chemical Engineering Company Limited,Dongguan 523581,China; 2Institute of Biochemical Engineering,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China; 3College of Urban and Civil Engineering,Shenzhen Graduate School of Harbin Institute of Technology,Shenzhen 518055,China; 4Bio-Treat Technology Company Limited,Dongguan 523581,China);Application of PCR-denaturing gradient gel electrophoresis (PCR-DGGE) in environmental microbial ecology.[J];Chinese Journal of Ecology;2006-10
5 WANG Fei1,YANG Guan-pin1,LI Xiao-jun2,LIU Wan2(1College of Marine Life Sciences,Ocean University of China,Qingdao 266003,Shandong,China;2Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China);Application of microbial markers in the researches of soil pollution ecology[J];Chinese Journal of Ecology;2008-01
6 Bu Yuanqing Huang Weiyi (Microbiology Department of College of Life Science of Nanjing Agricultural University, Key Laboratory ofMicrobiological Engineering of Agricultural Environment of Agricultural Ministry, Nanjing 210095, China)$$$$;EFFECT OF RICE STRAW ON MOLECULAR PHYLOGENETIC DIVERSIFY OF SOILBACTERIAL COMMUNITIES[J];Acta Pedologica Sinica;2005-02
7 Liu Wuxing~(1,2) Luo Yongming~(1,2) Teng Ying~(1) Li Zhengao~(1) Wu Longhua~(1)(1 Soil and Environmental Bioremediation Research Center,State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Sciences,Chinese Academy of Sciences,Nanjing 210008,China)(2 Graduate School of the Chinese Academy of Sciences,Beijing 100039,China);ECO-RISK & BIOREMEDIATION OF PETROLEUM CONTAMINATED SOIL Ⅰ.ISOLATION AND IDENTIFICATION OF PETROLEUM-EMULSIFYING BACTERIA[J];Acta Pedologica Sinica;2006-03
8 Shen Weishou~(1,2,3) Yin Rui~(1,2) Lin Xiangui~(1,2) Chu Haiyan~(1,2) Hu Zhengyi~(1) Cao Zhihong~(1,2)(1 Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China)(2 Joint Open Laboratory of Soil and Environment,Institute of Soil Science and Hongkong Baptist University,Nanjing 210008,China)(3 Graduate School of the Chinese Academy of Sciences,Beijing 100039, China);MICROBIOLOGICAL PROPERTIES OF AN ANCIENT PADDY SOIL DISCOVERED IN CHUODUNSHAN RELICS OF KUNSHAN,CHINA[J];Acta Pedologica Sinica;2006-05
9 Cai Xinde~(2) Qiu Rongliang~(1) Chen Guizhu~(1) Zeng Xiaowen~1 Fang Xiaohang~(1)(1 School of Environmental Science & Engineering,Sun Yat-Sen University,Guangzhou 510275,China)(2 South China Institute for Environmental Science,SEPA,Guangzhou 510655,China);RESPONSE OF MICROBIAL COMMUNITIES TO PHYTOREMEDIATION OF NICKEL CONTAMINATED SOILS[J];Acta Pedologica Sinica;2006-06
10 Li Hui~1 Zhang Ying~1 Su Zhencheng~1 Xu Hui~1 KRAVCHENKO Irina~2 Zhang Chenggang~1(1.Key Laboratory of Terrestrial Ecological Processes,Institute of applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China)(2.Winogradsky Institute of Microbiology,Russian Academy of Sciences,117312 Moscow.Russia);ANALYSIS OF BACTERIAL GENETIC DIVERSITY IN PADDY SOIL IN SHENFU IRRIGATION ZONE IRRIGATED WITH WASTEWATER FROM PETROLEUM INDUSTRY——BY DENATURING GRADIENT GEL ELECTROPHORESIS AND SEQUENCING OF PCRAMPLIFIED 16S RIBOSOMAL DNA FRAGMENTS[J];Acta Pedologica Sinica;2006-06
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Ruifu 1 Cao Hui 1,2 Cui Zhongli 1** Li Shunpeng 1 Fan Ben 1 ( 1Key Lab of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University,Nanjing 210095,China) ( 2Institute of Soil Science, the Chinese Academy of Sciences, Nanjing 210008, China);Extraction and Purification of Soil Microbial Total DNA[J];Acta Microbiologica Sinica;2003-02
2 ZHANG Huiwen,ZHANG Qianru,ZHOU Qixing,ZHANG Chenggang( Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016).;Introduction and progress of molecular microbial ecology.[J];Chinese Journal of Applied Ecology;2003-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Gong xian (Nanchang city Environmental Monitoring Station,Nanchang 330002);Assessment on Fluoride Pollution in Farm Siol and Water Samples in Nanchang City[J];Jiangxi Chemical Industry;2008-03
2 KUANG Shao-ping (College of Chemical and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042,China);Effect of Anion in Soils on Corns Lead Absorption[J];Journal of Qingdao University of Science and Technology;2003-03
3 Jiang Qiufeng (Chemistry Department of Qinghai Normal University,Xining 810008) Wang Zaimo (Northwest Plateau Institute of Biology,the Chinese Academy of Sciences,Xining 810001);Study on Selenium in Soils of Qinghai[J];;2000-01
4 JIANG Qiu feng (Department of Chemistry,Qinghai Normal University,Xining,810008);Influence of Selenium in Soil on Livestock Production and Preventing Measurement[J];CHINESE QHINGHAI JOURNAL OF ANIMAL AND VETERINARY SCIENCES;2000-03
5 CHEN Xuyu1) ZHOU Yakui1) ZHENG Fucong1, 2)(1 College of Environment and Plant Protection, Hainan University, Danzhou, Hainan 573717;2 Environment and Plant Protection Institute, CATAS, Danzhou, Hainan 573717);Comparison of Two Extracting Methods for Soil Microbial Total DNA[J];Chinese Journal of Tropical Agriculture;2008-03
6 Liao Xiangjun1,2 Ding Shijiang2 Wu Dan2 Feng Yasheng2 Zhang Benren1 (1 Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074;2 Hainan Bureau of Exploration and Development of Geology and Mineral Resources, Haikou 570206);Agro-geochemical Evaluation of the Agricultural Soils in the Northeast of Hainan Island[J];Chinese Journal of Tropical Crops;2005-02
7 Zeng Zhaohua (Institute of Environmental Geology, JBGMR,Nanchang,330012) Zeng Xueping (The Third People’s Hospital of Nanchang City, Nanchang,330009);RELATION BETWEEN CANCERS AND BORON IN SOIL IN CHINA[J];Acta Geologica Sichuan;2000-02
8 ZENG Zhao-hua1, ZENG Xue-ping2 (1.Institute of Environmental Geology, JBGMR, Nanchang, Jiangxi 330012; 2.The Third Hospital of Nanchang City, Nanchang, Jiangxi 330009);On Relationship between Cancer and Br Content in Soil[J];Acta Geologica Sichuan;2003-03
9 FU?Xian (Sichuan Agricultural Environmental Monitoring Station,Chengdu 610041 ,China);Status of Agricultural Soil and Development of High Quality and Safe Agricultural Products in Ebian County[J];Sichuan Environment;2005-03
10 PANG Xu-gui1,DAI Jie-rui1,XU Chun-mei2,HU Xue-ping1,ZHAO Xi-qiang1(1.Shandong Geological Survey Institute,Shandong Jinan 250013,China;2.Laizhou Bureau of Land and Resources,Shandong Laizhou 261400,China);Study on Geochemical Reference Value and Background Value of Soil in Pingyin County[J];Land and Resources in Shandong Province;2008-01
China Proceedings of conference Full-text Database 10 Hits
1 PENG jun Liang changcong ZHAO pei-jing HUANG jun-sheng~* Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou,Hainan 571737;Molecular Detection of Fusarium oxysporum f.sp.cubense in Infected Plant Tissue and Soil by Nested PCR[A];[C];2008
2 LIU Hong-ying~1 CHEN De-you~2 ZHOU Xue-mei~2 FENG Xiao-ming~1 GUO Kun-yi~1 (1.Nanjing Center,China Geological Survey,Nanjing Institute of Geology and Mineral Resources,Nanjing 210016,China; 2.Geological Survey of Sichuan Province,Chengdou,610213,China);Distribution characteristics of mercury in soils in Chengdou region and analyses on their influence factors[A];[C];2005
3 YUE Dan-ting, YANG Guo-Gang, LV Xin-Rong, ZHANG Rui-Shu (Marine Eng. College, Dalian Maritime Univ., Dalian 116026,China);Calculation of optimal oil heating time of oil tanker[A];[C];2007
4 DENG Jian, HUANG Li-wen, MOU Jun-min, ZHANG Jin-feng, and WEN Yuan-qiao;Implement of a Mesoscale Air-Sea-Wave Coupled Model System and Its Application in Environment Virtual Reality of Ship Navigation imulation[A];[C];2007
5 Wanghongzh, Zhangjundong;The Design of Marine Engine Room Monitoring and Controlling System[A];[C];2007
6 Feng Xue-jun, YUE Qiao-hong (Transportation and Logistics Engineering Institution of Hohai University, Nanjing 210098,China);Study on the Port Equipment scheme[A];[C];2007
7 Wang Qi, Duan Qingli, Huang Zhongliang (Jiangsu Maritime Instite 2111700 Nanjing MSA 210011);The necessity of the construction for the shipping companies' safty culture[A];[C];2007
8 WANG Yong, WANG Gui-jun, ZHANG Zhi-qiang, WANG Shu-xiao (Dep. of Navigation, Dalian Naval Academy, Dalian 116018, chian);Research on the security of Petroleum supply by ocean shipping[A];[C];2007
9 Gong Shaojun,(Nantong Vocation & Technical Shipping College, Nantong 226006, China) Huang Xiaobing(Jiangsu Far East Shipping Co., Ltd., Nanjing 210029, China);Introduction and application of card of radar video Acquisition and transaction[A];[C];2007
10 XU Jian,ZHU Fu-rong,LI Ming (Research center of Pesticides Guizhou University Guiyang 550025,China);Monitor and Evaluation of Soil Environmental Quality of Radix Pesudostellariae Base in Yuqing County Guizhou[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HU Pei~(1,2), PU Wan-fen~(2), HU Xing-qi~(2)(1.College of Chemistry, Sichuan Normal University, Chengdu 610066, China;2.Southwest Petroleum Institute, Chengdu 610500, China);Advances in Bioremediation of Petroleum-contaminated Soils[J];Leather Science and Engineering;2004-06
2 CHEN Yan, LI Guanghe, ZHANG Xu, LU Xiaoxia, ZHANG Ling(Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China);Effect of petroleum biodegradation and rhizosphere micro eco-system in phytoremediation of the polluted soil in oilfield[J];Journal of Tsinghua University(Science and Technology);2005-06
3 JIA Jianli, LI Guanghe, ZHANG Xu, LU Xiaoxia, DAI Dongjuan, ZHONG Yi(Department of Environmental Science and Technology, Tsinghua University, Beijing 100084, China;Microbial communities in petroleum polluted soils by PCR-DGGE[J];Journal of Tsinghua University(Science and Technology);2005-09
4 LUO Peng,HU Chao-qun,XIE Zhen-yu,ZHANG Lü-ping,REN Chun-hua,XU You-hou(The Key Laboratory of Applied Marine Biology of Guangdong Province and Chinese Academy of Sciences,South China Sea Institute of Oceanology,CAS,Guangzhou 510301,China);PCR-DGGE analysis of bacterial community composition in brackish water Litopenaeus vannamei culture system[J];Journal of Tropical Oceanography;2006-02
5 ZHENG Liangyong1) HU Jianfei1) LIN Changhua2)TANG Qunfeng2) GUO Qiaoyun3)(1 Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan 571737;2 College of Agriculture, SCUTA, Danzhou, Hainan 571737;3 Yuping Bureau of Agriculture, Yuping, Guizhou 554000);The Production of Succession Cropping Obstacles and Its Prevention and Cure Steps[J];Chinese Journal of Tropical Agriculture;2005-02
6 SANG Aiyun1) ZHANG Liming1) CAO Qimin1) XIA Weilin1) WANG Hua2) (1 Tropical Crops Genetic Resources Institute, CATAS, Danzhou, Hainan 571737; 2 College of Agronomy, SCUTA, Danzhou, Hainan 571737);Research Advances and Development Prospect of Phytoremediation in Heavy Metal Contamination Soil[J];Chinese Journal of Tropical Agriculture;2006-01
7 GUO Zhi-ping (Petrochemical College of Zhejiang Ocean University,Zhoushan 316004,China);The Prevention and Control of Oil Pollution off Chinese Coastal Waters[J];Journal of Zhejiang Ocean University;2004-03
8 LUO Xiao-mei,ZHANG Yi-rong,YANG Ding-qing (Analytical Center of Agricultural Academy of Sichuan Province, Chengdu 610066 ,China);Pollution Analysis and Assessment of Heavy Metals in Vegetables from Chengdu[J];Sichuan Environment;2003-02
9 LIU Guo-sheng~1, TONG Qian-ming~2, HE Chang-shun~1 , FAN Rui~1 (1.Zhuzhou Research Institute of Environmental Protection,Zhuzhou, Hunan 412000, China; 2.Hunan Research Institute of Territorial Resources,Changshang 410000, China);Investigation and Research of Cadmium Pollution in Soils[J];Sichuan Environment;2004-05
10 XIE Dan-ping~(1,2)(1.South China Research Institute of Environmental Sciences,SEPA,Guangzhou 510655,China; 2.Department of Environmental Engineering,Jinan University,Guangzhou 510632,China);Research Advances on Bioremediation of Crude Oil Pollution[J];Sichuan Environment;2006-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Chunhai Zhao,Xuri Tang(Biological Departmentof Binzhou vocational college Binzhou 256624 China)(Biology Science and Engineering Academy,Hebei University of Economics and Business,Shi Jia Zhuang 050061);Heavy-Metal-Pollution of soil and remediation of polluted soils[J];Life Science Instruments;2007-04
2 QIN Qing,LI Hai-xia,WANG Jun-wei,WAN Xu(Sichuan Provincial Environmental Monitoring Center,Chengdu 610041,China);Determination of Cu,V,Ni and Cr in Soil by the Technology of Inductively Coupled Plasma Atomic Emission Spectrometry[J];Sichuan Environment;2008-05
3 LI Hong-liang1,MA Ben-sheng2 (1.Department of Chemistry,Shandong Normal University,Jinan Shandong 250014,China;2.Xusheng School of Shuangshan Office,Zhangqiu Shandong 250200,China);Application of the phase inversion of microemulsions in the organic contaminant separation[J];Shanxi Chemical Industry;2008-02
4 SHEN Wei-Shou1,2,3, YIN Rui1,2, LIN Xian-Gui1,2,, CHU Hai-Yan1,2, HU Zheng-Yi1,2, CAO Zhi-Hong1,2 1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 2 Joint Open Laboratory of Soil and the Environment, Institute of Soil Science and Hong Kong Baptist University, Nanjing 210008, China3 Graduate University of Chinese Academy of Sciences, Beijing 100049, China;PCR-DGGE analyses of bacterial and archaeal community diversities in ancient paddy soils discovered in Chuodunshan Site, Suzhou, China[J];Acta Ecologica Sinica;2008-06
5 CHEN Min-Ling,LI Wei-Hua,CHEN Zhang-He Key Laboratory of Ecological and Environmental Sciences in Guangdong Higher Education,College of Life Science,South China Normal University,Guangzhou 510631,China;Methods for investigation of microbial diversity on different levels:a review[J];Acta Ecologica Sinica;2008-12
6 QIN Yu-jie,ZHOU Shao-qi(College of Environmental Science and Engineering,South China University of Technology,Guangzhou 510641,China);Research progress on anaerobic ammonium-oxidation bacteria[J];Chinese Journal of Ecology;2007-11
7 JIANG Xin1,2, MA Ming-chao1, LI Jun2, ZHONG Zuo-shen1 (1School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China; 2Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China).;Molecular ecology of anammox bacteria in constructed rapid infiltration system[J];Chinese Journal of Ecology;2008-04
8 Qihui Teng1, Hui Cao1*, Zhongli Cui1,2, Ying Wang1, Bo Sun2, Hongtao Hao2, Shunpeng Li1 1 Key Laboratory of Microbiological Engineering of Agricultural Environment, MOA, Nanjing Agricultural University, Nanjing 210095 2 Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008;PCR-RFLP analysis of bacterial 16S rDNA from a typical garden soil in Taihu region[J];Biodiversity Science;2006-04
9 HU Yuan-sen~1,LI Cui-xiang~1,ZHOU Yi~2,WANG Ya-guo~1 (1.College of bioengineering,Henan Industrial University,Zhengzhou 450052,China;2.Henan Shenghua bioengineering limited corporation,Xinyang 464000,China);Comparison of Two DNA-Extraction from Soil[J];Biotechnology;2006-05
10 Zhou Lin1,3 Zhang Xiaojun2 Li Guoxun1 Zhang Jie3** (1 College of Plant Protection,Agricultural University of Hebei,Baoding 071000;2 College of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240;3 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094);The Application of DGGE/TGGE Techniques in Molecular Microbial Ecology of Soil[J];Biotechnology Bulletin;2006-05
China Proceedings of conference Full-text Database 4 Hits
1 Bi Mingli Yu Wantai (Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China);Microbial Technology and Application in Agro-ecosystem[A];[C];2008
2 WU Yin-Bao, Shi JingCai, Lan XiaoHua, Chen WengYan (College of Animal Science, South China Agricultural University, Guangzhou 510642, China);Comparison of Methods for DNA Extraction from pig Feces and Soil Microorganism[A];[C];2006
3 (1.Research Institute of Forest Ecology,Environment and Protection,Key Laboratory of Forest Protection,State Forestry Administration,CAF,Beijing 100091,China;2.College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,Hubei,China);Relationship Between the Number of Microbial and Soil Properties of typical vegetation in Three Gorges Reservoir Area[A];[C];2009
4 TANG Jing-chun,SUN Qing (College of Environmental Science and Engineering,Nankai University; Key Laboratory of Pollution Processes and Environmental Criteria,Ministry of Education,Tianjin 300071,China);Management on the Bioremediation of Petroleum Contaminated Soil and its Cost Analysis[A];[C];2009
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