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《Ecology and Environmental Sciences》 2010-04
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The application of molecular techniques to characterize the microbial communities in constructed wetland

LIANG Wei1,WU Suqing1,2,WU Zhenbin1 1.State Key Laboratory of Freshwater Ecology and Biotechnology//Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China.2.Graduate University of the Chinese Academy of Sciences,Beijing 100049,China  
The studies and applications of constructed wetland have become a hot topic in recent years.Microorganism is one of the most important factors in constructed wetland system.The structure of microbial communities have great influence on the purifying capacity of constructed wetland.Compared with traditional microbial analysis technologies,molecular biological techniques have many advantages,such as cultivation-independent,efficient,fast and simple,which have been widely used to identify environmental microorganisms.In the paper,the molecular biological techniques based on polymerase chain reaction(PCR),such as PCR-DGGE,LH-PCR,T-RFLP,PCR-SSCP and ARDRA,were introduced.The mechanisms and research advances of the molecular techniques were also summarized.By using these techniques,the microbial number,density,diversity and dominant species in the constructed wetland systems treating with specific wastewater(such as swine wastewater,landfill leachates,etc.) can be analyzed;the relationship between the number,activity distribution,space shifting of specific microbial functional group(such as ammonia-oxidizing bacteria,denitrifiers,sulfate reducers,etc.) and the removal rates of main pollutants can be identified;the influence on microbial diversity and stability under different conditions(such as different substrate,wetland plants,hydraulic loading,etc.) can be estimated.Finally,the research prospects for the molecular techniques in the constructed wetland were also proposed.
【Fund】: 国家重大科技专项(2008ZX07106-003 2008ZX07316-004);; 江苏省科技惠民项目(BE2008651);; 中科院天津专项(TJZX2-YW-07)
【CateGory Index】: X172
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