Construction of eukaryotic expression vector of tumor suppressor gene FHIT

ZHU Chang jun, QU Xun, SONG Wen gang, MA Shi bin, DENG Wen(Dept. of Immunology, Taishan Medical College, Taian 271000,China)  

Objective: To construct an eukaryotic expression vector of tumor suppressor gene FHIT. Methods: The full length cDNA fragment of FHIT was obtained by the digestion of cloning vector pGEM FHIT with EcoRI,then cloned into eukaryotic expression vector pHCMVsp1A under the CMV promoter.The recombinant plasmid DNA was identified by the digestion of HindIII and PCR. Results: The FHIT cDNA was cloned into the pHCMVsp1A in the right direction. Conclusion: The eukaryotic expression vector pHCMVsp1A FHIT has been constructed successfully and it may be used for further research on the tumor suppressor function of FHIT.

【Key Words】： FHIT suppressor gene eukaryotic expression
【CateGory Index】： R346

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