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《Journal of Oceanography in Taiwan Strait》 2010-01
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Simple methods for screening the oceanic oleaginous microorganisms

QIN Yan-hong,YE De-zan(Key Laboratory of Marine Biogenetic Resource/Third Institute of Oceanography,SOA,Xiamen 361005,China)  
Two methods for rapid screening intracellular lipid content in oceanic oleaginous microorganisms (28 yeasts and 143 moulds) were established using a fluorescent probe Nile red in this paper.The first method screened oceanic oleaginous microorganisms using Nile red,established by the Nile red directly included in the medium and then compared the fluorescence intensity under ultraviolet.2 white yeasts out of 28 yeasts and 7 non-black moulds out of 143 moulds were prominent in producing intracellular fatty acid.However,this method was not suitable for red yeasts(17) and black moulds(28).The second method,by comparing the fluorescence intensity per OD600,one reddish yeast was selected with much higher fluorescence intensity.However,this method could not apply to moulds due to their inter-twisted mycelia.With above methods most of screened microorganisms capable of producing high content intracellular fatty acid are from deep sea.
【Fund】: 中国大洋协会资助项目(DY105-02-03 DY-105-04-02-10)
【CateGory Index】: Q938
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