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《Journal of Taizhou University》 2014-06
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Cloning and Sequence Analysis of Malate Dehydrogenase Gene from Arachis hypogaea L.

WANG Qiao-ying;YING Hao-ze;YU Jia-ning;ZHU Jin-jing;XU Zi-li;DU Zhao-kui;School of Life Sciences, Taizhou University;Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation;Institute of Ecology, Taizhou University;  
In this paper, degenerate primers were designed according to reported malate dehyrogenase gene sequences. The coding sequence of malate dehyrogenase gene(Ah MDH) numbered KF499119 was amplified by RT-PCR from c DNA of Arachis hypogaea L. The sequence of Ah MDH is 1071 bp in length encoding 356 amino acid. Multiple sequence alignment analysis showed that A. hypogaea, Glycine max, Medicago truncatula and Pisum sativum share high homology.
【Fund】: 浙江省教育厅科研项目(Y201223322);; 2014年浙江省大学生科技创新活动计划(新苗人才计划)(2014R428013)
【CateGory Index】: S565.2
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