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《Journal of Tianjin University of Traditional Chinese Medicine》 2013-04
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Effect of magnetic fields on human brain microvascular endothelial cell proliferation under normal and hypoxia conditions

MENG Qing-nan;WANG Yun-hua;ZHANG Hao-nan;YANG Lin;WANG Yi-min;Traditional Chinese Medicine Engineering Institute of Tianjin University of TCM;Tianjin University of TCM;  
[Objective] To observe effect of different permanent magnetic fields on human brain microvascular endothelial cell(HBMEC) proliferation under normal and hypoxia conditions. [Methods] HBMEC were cultured in vitro under normal condition and hypoxia condition, experimental cells were divided into 10 groups, control group,ginkgo biloba injection(350 mg/L) group, different induction magnetic field groups(3.4, 5.6, 13.8, 27.8, 52.6, 107.0,178.9, 292.2 mT), each group was collected specimens 48 h after planking, MTT method was used to measure cell proliferation. [Results] Compared with control group, the drug group was statistically different(P 0.05) under normal condition and hypoxia condition, magnetic field groups were no statistically different(P0.05); there was no statistical difference between magnetic field groups(P 0.05). [Conclusion] Magnetic field groups with magnetic induction 3.4,5.6,13.8,27.8,52.6,107.0,178.9,292.2 mT have no effect on proliferation of HBMEC cultured under normal or hypoxia condition.
【Fund】: 国家自然科学基金资助项目(30873467)
【CateGory Index】: R363
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