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《Chinese Journal of Inorganic Chemistry》 2009-02
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Interaction of Pb(Ⅱ) with Bovine Serum Albumin under UV Irradiation

ZHANG Hai-Rong1 BIAN He-Dong*,1 NI Shou-Hai1 YU Qing 1 LIANG Hong*,2 CHEN Zhen-Feng2(1College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin,Guangxi 541004)(2Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources(MOE),Guangxi Normal University,Guilin,Guangxi 541004)  
The interaction between Pb(Ⅱ)and bovine serum albumin(BSA)under the effect of UV C(253.7nm)irradiation at physiological condition have been investigated by UV spectrum,ultraviolet second-derivative spectroscopy and fluorescence spectrum.The research results indicated that UV C irradiation make the environments of aromatic residues change according to UV spectrum and ultraviolet second-derivative spectroscopy.Stern-Volmer equation and Lineweaver-Burk equation showed that the fluorescence quenching of BSA by Pb(Ⅱ)is also a static quenching procedure and strong binding site is not change after UV C irradiation.When adding Pb(Ⅱ)to irradiated BSA,the binding constant(KS)decreased gradually;while irradiating the mixture of Pb(Ⅱ)-BSA,the binding constant(KS)increased.
【Fund】: 国家自然科学基金(No.20671023 30460153);; 教育部重点项目基金(No.03101 204111);; 药用资源化学与药物分子工程教育部重点实验室的资助项目
【CateGory Index】: Q51
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