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《Medical Journal of the Chinese People's Armed Police Forces》 2009-02
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Protective effect of ischemic postconditioning on intestinal ischemia-reperfusion injury and its mechanism

LIU Yuhui,and ZHANG Hongying.Department of General Surgery,The Third Hospital of Beijing Municipal Corps,Chinese People's Armed Police Forces,Beijing 100039,China  
Objective To study the protective effects of intestinal ischemic postconditioning(I-postC) on ischemia-reperfusion(IR) injury and its mechanism.Methods Seventy-two Spraque-Dawley rats were divided into three groups at random:control group,IR group and I-postC group.Each group was subdivided into 10,45 and 90min reperfusion subgroups(n=8).The activity of diamide oxidase(DAO) was measured by spectrophotometry.The activity of superoxide dismutase(SOD) was measured by xanthine oxidase method.The content of maleic dialdehyde(MDA) was measured by thiobarbituric acid chromatometry.The content of myeloperoxidase(MPO) was measured by myeloperoxidase method.The content of nitric oxide(NO) was measured with nitrate reductase method.The expression of nitric oxide synthase(iNOS) in intestine were detected by real-time quantitative PCR(RT-PCR).Results(1) Compared with IR group,DAO in the intestinal tissue markedly increased(P0.05) and DAO in the plasma significantly decreased at 45 min and 90 min after reperfusion in I-postC group(P0.05).(2) Compared with IR group,MDA and MPO in the intestinal tissue significantly decreased at 10,45 and 90 min after reperfusion in I-postC group(P0.05),and intestinal SOD activity markedly increased at 45 min and 90 min in I-postC group(P0.05).(3) Compared with IR group,I-postC significantly lowered the levels of NO in the plasma and iNOS mRNA expression at 45min and 90min(P0.05),while level of NO in the plasma in IR group was higher than in I-postC group at 10min after reperfusion(P0.05).Conclusions The intestinal injury following IR is related to the disturbance of NO and elevated expression of iNOS.The protecting effects of I-postC might be induced by earlier but lower NO levels and it's protective effects.
【CateGory Index】: R363
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Chinese Journal Full-text Database 2 Hits
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