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《Medical Journal of the Chinese People's Armed Police Forces》 2009-02
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Protective effect of ischemic postconditioning on intestinal ischemia-reperfusion injury and its mechanism

LIU Yuhui,and ZHANG Hongying.Department of General Surgery,The Third Hospital of Beijing Municipal Corps,Chinese People's Armed Police Forces,Beijing 100039,China  
Objective To study the protective effects of intestinal ischemic postconditioning(I-postC) on ischemia-reperfusion(IR) injury and its mechanism.Methods Seventy-two Spraque-Dawley rats were divided into three groups at random:control group,IR group and I-postC group.Each group was subdivided into 10,45 and 90min reperfusion subgroups(n=8).The activity of diamide oxidase(DAO) was measured by spectrophotometry.The activity of superoxide dismutase(SOD) was measured by xanthine oxidase method.The content of maleic dialdehyde(MDA) was measured by thiobarbituric acid chromatometry.The content of myeloperoxidase(MPO) was measured by myeloperoxidase method.The content of nitric oxide(NO) was measured with nitrate reductase method.The expression of nitric oxide synthase(iNOS) in intestine were detected by real-time quantitative PCR(RT-PCR).Results(1) Compared with IR group,DAO in the intestinal tissue markedly increased(P0.05) and DAO in the plasma significantly decreased at 45 min and 90 min after reperfusion in I-postC group(P0.05).(2) Compared with IR group,MDA and MPO in the intestinal tissue significantly decreased at 10,45 and 90 min after reperfusion in I-postC group(P0.05),and intestinal SOD activity markedly increased at 45 min and 90 min in I-postC group(P0.05).(3) Compared with IR group,I-postC significantly lowered the levels of NO in the plasma and iNOS mRNA expression at 45min and 90min(P0.05),while level of NO in the plasma in IR group was higher than in I-postC group at 10min after reperfusion(P0.05).Conclusions The intestinal injury following IR is related to the disturbance of NO and elevated expression of iNOS.The protecting effects of I-postC might be induced by earlier but lower NO levels and it's protective effects.
【CateGory Index】: R363
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Yao Shutong, Li Yuzhen, Liu Xiuhua, et al/Department of Pathophysiology, PLA General Hospital, Beijing 100853;Effect of Ischemic Postconditioning on Mesenteric Microcirculation of Rats Subjected to Small Intestinal Ischemia/Reperfusion Injury[J];Chinese Journal of Microcirculation;2007-02
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZENG Yuan-Feng, XIAO Yi-Sheng, ZHU Qing-Xian , SHAO Li-Jian (Department of Pathology, the First Affiliated Hospital of Nanchang University , Nanchang 330006, China; Department of Morphology, Jiangxi Traditional Chinese Medical College ; Department of Histology and Embryology , Medical College, Nanchang University. Department of Anatomy , Medical College , Nanchang University);Protective effects of small intestinal ischemic preconditioning on ischemia/reperfusion injury and its mechanism[J];Chinese Journal of Anatomy;2006-05
2 LIU Ke-xuan,HUANG Wen-qi;Protective effects of preconditioning and postconditioning against the intestines I/R injury[J];China Continuing Medical Education;2010-04
3 CHEN Yaoming,Baozhen People's Hospital,Chongming County,Shanghai,2021157;Analysis on Etipathogenisis of Lower Gastrointestinal Hemorrhage[J];Journal of Military Surgeon in Southwest China;2007-05
4 Ban Runwu, et al. (Department of General Surgery, the Fifth Affiliated Hospital to Harbin Medical University, Daqing Heilongjiang 163316China);Protective Effects of Ginko Biloba Extract on Multiple Organ Injury Induced by Hepatic ischemia-reperfusion in Rats[J];Journal of Qiqihar Medical College;2007-01
5 WANG Hai-qing,OUYANG-jun(Xinjiang shihezi university Xinjiang shihezi 832000 China);Effect of different fluid resuscitation on intestinal mucosa in rats with hemorrhagic shock[J];Progress in Modern Biomedicine;2011-03
6 Zhao Xia,Dong Shuyun,Zhang Lianyuan North China Coal Medical college,Tangshan 063000;The Changes of Mesentery Microcirculation in Rats with Hind Limbs Ischemia-Reperfusion[J];Chinese Journal of Microcirculation;2010-01
7 Zhen Li,Jun Ouyang,Department of Emergency Surgery,the First Affiliated Hospital,Medical School of Shihezi University,Shihezi 832000,Xinjiang Uygur Autonomous Region,China;Hypertonic sodium chloride hydroxyethyl starch injection reduces histopathological changes in the intestinal mucosa of rats with hemorrhagic shock[J];World Chinese Journal of Digestology;2012-12
8 WANG Duo-jun.(Department of Senate Bureau,the Second Affiliated Hospital of Mudanjiang Medical College,Mudanjiang 157009,China);Colonoscopy under the Emergency Diagnosis and Treatment of 56 Cases of Gastrointestinal Bleeding of Clinical Experience[J];Medical Recapitulate;2009-01
9 YAO Shu-tong1,2,LIU Xiu-hua1,TANG Xu-ming1,SUN Sheng1,WANG Jia-fu2(1Department of Pathophysiology,Chinese PLA General Hospital,Beijing 100853,China; 2Department of Pathophysiology,Taishan Medical College,Taian 271000,China.;Ischemic postconditioning ameliorates pia mater microcirculation in rats subjected to cerebral ischemia reperfusion[J];Chinese Journal of Pathophysiology;2009-03
10 YAN Chao-qi~1,ZOU Xiao-ming~1,LI Gang~1,SONG Mao-li~1,LI Xiao-lin~1,LI Yun-long~1, YANG Chun-fa~1,JIANG Ying~1,LI Dong-mei~1,ZHOU Ya-bin~2(1.The Second Affiliated Hospital of Harbin Medical University,Harbin 150086, China;2.The Heilongjiang University of Chinese Medicine,Harbin 150040,China);Perfusion and Preservation of Isolated Piglets Small Intestine Graft for Transplantation[J];Chinese Journal of Comparative Medicine;2008-12
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