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Extrinsic Gene Expression in the Skeletal Muscle of the Rabbit Introduced by Direct Injection

Shi Yaxue,He Tianyuan, Zhang Baigen, et al. Division of Vascular Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai(200001)  
Objective: To explore whether extrinsic gene injected directely into the skeletal muscle of the rabbit can be expressed injected directly and to define the effect of various conditions such as volume of fluid and DNA dosage on the gene expression. Methods: p-galactosidase gene was used as a reporter gene to be transfered into skeletal muscle by direct injection. Various conditions such as volume of fluid (50μl, 100μl, 150μl) and DNA dosage (10μg, 25μg, 50μg, 80μg, 100μg) were studied for their effect on the level of β-galactosidase activity expressed from the plasmid DNA. Results: The volume injected had no effect on the level of gene expression, but did influence the stability of expression. The level of expression increased in proportion to the dosage used. Conclusions: Extrinsic gene could be expressed in skeletal muscle cells by direct injection of the gene and the expression level increased with the volume and dosage used. Direct gene injection may be a novel gene delivery method to treat lower limb vascular occlusion disease.
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