Studies on the stimulating reaction of pituitary and ovary to sexual hormones in Cadmium-induced rats
ZHANG Wen-chang, WU Zhi-ren, LI Huang-yuan, et al. (The School of Public Health, Fujian Medical Uniuersity, Fuzhou 3500041, China)
Objective To investigate the effects of cadmiun (Cd)on female gonads, especially the stimulating reaction of pituitary and ovary to sexual hormones ; Methods SD female rats were given subcutan eously with the different doses of Cd for 6 weeks(5 days a week, daily) and the estrus cycle of rats was observed, the changes of Cd levels in serum, ovary and uterus were measured, respectively, the gonadotrpic-release hormons (GnRH)stimulating test were carried out in rats, with the serum FSH and LH level determined.30 female mice(KM) were assigned randomly to 3 groups and given the different doses of Cd intraper-itoneally for 5 days (daily) and then the super-ovulation test was perfoned, Results (1)the Cd comtents both in the uterus and the ovary of Cd rats were higher(P0.05);(2)the ratio of estrus cycle abnormity was higher in the Cd rats than in the control;(3)before the injection of GnRH, there were not significant difference of serum FSH, LH between groups, After the injection of GnRH, the level of serum FSH and LH increased significantly in the Cd rats than in the control(P0.05);(4)in the super-ovulation test, we had not found the signficant difference of the amount of ova between each groups . Conclusion The ovary may be an important target of Cd effect,causing the gonad toxicity of female rats, such as the abnormity of estrus cycle, Both the lstmulating reaction function and the compensation ability of pituitary to the sexual hormones have been damaged, but no damage was found as regards ovary to super level sexual hormones has not been found.