The activation of primary astrocytes induced by manganese chloride
DONG Li-sha;LI Xue-hui;LIU Wen-li;ZHAO Peng;YAO Bi-yun;ZHOU Zong-can;School of Public Health,Peking University;
Objective To explore the influence of manganese to astrocyte cells. Methods Primary astrocytes were dissociated from newborn rat,and identified with antibody of glial fibrillary acidic protein( GFAP),which is specific protein expressed in astrocytes.Astrocytes were treated with different concentrations of manganese chloride( MnCl_2) for 24 h. The survival rate of astrocytes were detected with MTT colorimetry test. The cell areas and expression of GFAP were measured by high content analyzer,and the ROS was determinated with flow cytometry. Results After 24 h treated with different concentrations of MnCl_2,the survival rate of astrocytes decreased dosedependently,the areas of astrocytes were reduced,the expression of GFAP and the ROS increased. Conclusion Manganese chloride induces the activation of astrocytes.