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《MICROBIOLOGY》 1999-01
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PREPARATION AND REGENERATION OF PROTOPLASTS OFCURVULARIA LUNATA

Wang Deng, Du Lianxiang(Food Engmeering Dept. Tianjin University of Light Industry, Tianjin 300222)  
This Paper studies the conditions of preparation and regeneration of Cuvrularia lunata. The myceliawere cultured for 16h and suspended in 0.6mol/L KCI, after dealing by DTT for a short time, then Lywallzyme(1%) and Cellulase (1%) were added. The forming number of protoplasts was 6.0× 106 / mol and theregeneration frequency was 8.3%.
【Fund】: 天津市科技攻关资助
【CateGory Index】: Q93
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