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《Microbiology》 2002-05
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OPTIMIZATION OF BIOPROCESS OF CATALASE PRODUCTION BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY

LIU Jian-Zhong 1 WENG Li-Ping 1,2 JI Liang-Nian 1,2 (The Key Laboratory of Gene Enigineering of Minstry of Education, Zhongshan University, Guangzhou 510275) 1 (Department of Chemistry, Zhongshan University,Guangzhou 510275) 2  
Response surface methodology is the most widely used statistical technique for bioprocess optimization. It has been succesfully employed for optimizing the medium integrants and operation conditions in many fermentations. The interaction among the possible influencing parameters can be evaluated with limited number of experiments by using response surface methodology. Response surface methodology was applied to optimize the speed of agitation and the rate of aeration for the maximum production of catalase by Aspergillus niger . A 2 2 central composite design using response surface methodology was employed in this investigation. A quadratic model for catalase production was obtained. The maximum level of catalase was achieved when the speed of agitation and the rate of aeration were 691 r/min and 1.3 L·L -1 ·min -1 .
【Fund】: 广东省自然科学基金 (No.0 0 1 2 78);; 高等学校重点实验室访问学者基金资助项目
【CateGory Index】: TQ925
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