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《Microbiology》 2003-05
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STUDY ON BIOSYNTHESIS PATHWAYS OF A NTIB IOTIC AGPM

CHEN Gui-Bin NIU Jin-Yang ZHANG Jian-Yon g YUAN Yin-Jin HU Zong-Ding (Department of Pharmaceutical Engineering, School of Chemical Engineering, Tianjin University, Tianjin 300072)  
A new antitumor antibiotic AGPM biosynthesis pathways was studied with feeding e xperiment, resting cell system and enzyme inhibitor experiments. The results showed that addition of the amino acids and branched fatty acids such as Ile, Val, Met, Glu and acetate, propionate and butyrate, which could be transfo rmed to active precursors of polyketide, could effectively improve the productio n of antibiotic AGPM. Cerulenin(25μg/mL) and iodoacetamide (0.5mmol/L)-s peci fic inhibitors of polyketide synthase and fatty acids synthase could decrease an tibiotic production to 35.3% and 26.2% that of control respectively, without a ffecting cell growth. When idioacetamide concentration increased to 1.0mmol/L, antibiotic AGPM production would be fully inhibited. All these results testifi ed antibiotic AGPM was biosynthesized by polyketide pathway.
【Fund】: 国家高技术研究发展计划 ( 86 3)项目(No 2 0 0 1AA2 14 0 81)~~
【CateGory Index】: Q933
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