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《Microbiology》 2005-02
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The Selection,Identification and Characteristic Study on a Host Strain of Lactic Acid Bacteria

WANG Yin-Yu 1,2 CHEN Li-Shan2 JIA Shi-Fang3 REN Da-Ming2 GUO Ben-Heng 1*(Technical Center, Bright Dairy & Food Co, Ltd, Shanghai 200072)1 (State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433)2 (Institute of Microbiology, The Chinese Academy 0f Sciences, Beijing 100080)3  
As a host strain, Lactic acid bacteria COCC101 strain, isolated from a capsule, was identified as Enterococcus faecali. The drug-resistance experiment indicated that it is sensitive or in medium sensitive to most of drugs. There is no plasmid in this strain. The transformation efficiency is in direct proportion to the voltage. The highest efficiency is 2×104 transposons/μg DNA. The strain can accept plasmids derived from different origin, and it expressed heterogeneous Green Fluorescent Protein (GFP) under the Nisin induction. All of these results indicated that the COCC101 strain is hopeful to be a host strain.
【CateGory Index】: Q939.97
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