Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Microbiology》 2009-06
Add to Favorite Get Latest Update

Identification of Antagonistic Strain P-13 and the Preliminary Studies on Its Antimicrobial Substances Against Bacterial Spot Diseases of Muskmelon

YANG Liang1,2 SHI Ying-Wu2 WU Zhong-Hong2,3 LOU Kai2 (1. College of Food, Shihezi University, Shihezi, Xinjiang 832003, China) (2. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 830091, China) (3. College of Food Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China)  
An actinomycete strain P-13, with antimicrobial activity against muskmelon bacterial spot pathogens, was isolated from the muskmelon rhizosphere soil samples in Xinjiang. The strain P-13 was identified as Streptomyces rochei based on morphological, physiological characteristics and 16S rRNA sequence analysis. The agar diffusion bioassay showed that the diameter of inhibition zone against Acidovorax avenae subsp. citrull BFB and Pseudomonas syringae pv. Lachrymans P4 was above 19 mm and 17 mm, respectively. The antimicrobial substances obtained from strain P-13 were demonstrated to be alkaline and water-soluble compounds according to paper chromatogram analysis and exocellular metabolites. Furthermore, it was stable to be treated by 100°C for 10 min, pH 6 for 6 h, or ultraviolet treatment for 7 h. Moreover, it was insoluble in organic solvents, such as petroleum benzine, diethyl ether, and acetic ether.
【Fund】: 农业部948项目(No2006-G62)
【CateGory Index】: S436.5;S476
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 7 Hits
1 LI Jing1, AN De-rong1, LIU Cui-juan1, ZHANG Qin-fu2, XIE Yi2, TIAN Xuan3 (1.College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling 712100, Shaanxi, China; 2.Shaanxi Hailang Institute of Biological, Xian 710000, China; 3.State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China);A Preliminary Study on the Inhibitory Substances of Actinomycete S-159-05[J];Agrochemicals;2007-11
2 CHEN Yi-Guang~(1,2)ZHANG Xiao-Rong~1 ZHANG Li~1 LIU Zhu-Xiang~1 PENG De-Jiao~1 XIAO Huai-Dong~1 HUANG Ke~1 CHEN Qi-Hui~1 XU Li-Hua~(2*) (1.College of Bio-resources and Environmental Science,Institute of Ecology,Jishou University,Jishou,416000) (2.Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-resources, Yunnan University,Kunming,650091);Isolation and Identification of Marine Actinomycete JMC06001 Exhibiting Strong Antibacterial Activity[J];Microbiology;2008-01
3 Lin Ma,Hongbing Chen,Jucai Han,Huiping Liu (College of Agriculture,Shanxi Agricultural University,Taigu 030801,China);Identification of endophytic actinomycete Lj20 from plant and its antifungal substances[J];Acta Microbiologica Sinica;2008-07
4 SHAO Yan-po,FANG Li-ping,WEI Shao-peng,LI Hui-ling and JI Zhi-qin (Institute of Pesticide Science,Northwest A & F University,Yangling Shaanxi 712100,China);Studies on Antagonistic Spectrum and Stability of Actinomycete B5-strain from Marine[J];Acta Agriculturae Boreali-Occidentalis Sinica;2007-03
5 Xu Ping 1,2, Li Wen-jun 1, Gao Hui-ying 2, Sun Yu-min 2, Zhang Hua 2, Xu Li-hua 1, He Jian-gong 2 and Jiang Cheng-lin 1 (1 The Key Laboratory for Microbial Resources of Ministry of Eductation, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091; 2 New Drug R & D, North China Pharmaceutical Corp., Shijiazhuang 050015);Molecular screening and distribution of aromatic polyketide antibiotics producers from Actinomycetes[J];Chinese Journal of Antibiotics;2005-03
6 CHEN Li-li, GAO Bi-da (College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha 410128, China);Progress of the Research on Microbial Metabolites against Plant Virus Diseases[J];Chinese Journal of Biological Control;2006-04
7 Sui Qin1,2, Liu Weicheng2, Qiu Jiyan2, Liu Ting2, Pan Zhengyan1, Liu Xuemin1(1.Department of Plant Protection,Northeast Agricultural University,Harbin 150030,China; 2.Institute of Plant & Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China);Determination on the antimicrobial spectrum and the stability of the antagonistic activity of Streptomyces lydicus A02[J];Plant Protection;2007-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 XING Kun et al (Department of Pesticide Science, College of Agronomy, Shanxi Agricultural University, Taigu,Shanxi 030801);Identification and Antagonistic Effect of Endophytic Actinomycetes Isolated from Capsicum[J];Journal of Anhui Agricultural Sciences;2005-05
2 ZHANG Chao,CAO Hai-qun,CHEN Li,WANG Jie (College of Plant Protection,Anhui Agricultural University,Hefei 230036);Preliminary Study on the Inhibition of Polysaccharide of Edible Fungi to Plant Virus[J];Journal of Anhui Agricultural University;2005-01
3 PAN Zheng-yan~(1),LIU Wei-cheng~(2),QIU Ji-yan~(2),DONG Ke-yu~(2),TIAN Zhao-feng~(2),LIU De-wen~(2),LIU Xue-min~(1)(1.Northeast Agricultural University,Harbin 150030,China;2.Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100089,China);Control Effect of the Actinomyces Strains Ⅲ-61 and A-21 to Fusarium Wilt and Gray Mold of Vegetables[J];Acta Agriculturae Boreali—Sinica;2005-04
4 ZHENG Yaotong~(1,2), LING Qiying~2, XIE Lianhui~2(1. College of Resources and Environment, Fujian Agriculture and Forest University, Fuzhou350002; 2. Institute of Plant Virus,Fujian Agriculture and Forest University,Fuzhou);Removal and inactivation of TMV by bacteria-algal ecosystem in a wastewater stabilization pond[J];Acta Scientiae Circumstantiae;2004-06
5 LIN Qin-xiong,XI Tao,LIU Chun-hui,GU Jun,XING Ying-ying(School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China);Preliminary research of bioactive metabolite produced by marine actinomyces M326[J];Chinese Journal of Marine Drugs;2006-01
6 DING Lixiao, WANG Xin, WANG Dongchang, FANG Shankang (Department of Plant Protection, Laiyang Agricultural College, Laiyang Shandong 265200);Studies on the Agricultural Antibiotic on Controlling Tobacco Brown Spot[J];JOURNAL OF JILIN AGRICULTURAL UNIVERSITY;1999-02
7 Zhu Changxiong;Zhongshengmycin, a New Agro-antibiotics[J];Fine and Specialty Chemicals;2002-16
8 ZHANG Cheng 1,XIN Yu-cheng 2,ZHAO Su-xiang 3,LIU Yuan-mei 4,WANG Cun-li 5 (1. Agric. Bureau of Gaomi, Gaomi 261500,China; 2. Laiyang Agri. Collage; 3. Zijing Town of Penglai; 4. Agric. Bureau of Changle; 5. Yinma Town of Changyi);Application and Purification of the Antivirus of Penicillin sp.[J];Journal of Laiyang Agricultural College;2002-02
9 Ma Lin, Han Jucai, Liu Huiping (Department of Pesticide Science, College of Agriculture, Shanxi Agricultural University, Taigu Shanxi 030801 );Inhibiting Effect of Endophytic Actinomycetes Fq24 and Lj20 on Alternaria solani (Ell. et Mart) Jones et Grout[J];Pesticide Science and Administration;2006-05
10 Zhang Rongyi; Tan Zhiqiong; Wen Yantang; Zhang Huimei; Jiang Riming(Dept. of Plant Protection,College of Agronomy,SCUTA,Danzhou Hainan 571737);Description and Identification of the Causal Organism of Bacterial Fruit Blotch of Watermelon[J];CHINESE JOURNAL OF TROPICAL CROPS;1998-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved