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《Journal of Microbiology》 2002-03
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MOLECULAR DETECTION OF HOMOLOGY OF HYDROGEN UPTAKING GENE IN Azospirillu brasilense

LUO Yonghua et al. (Guangdong Inst. of Microbiol. Guangzhou 510070)\;  
Azospirillum brasilense strains capable of producing hydrogen uptaking enzyme protein (Hup\++) were screened through TTC (2,3,5 triphenyl tetrazolium chloride) experiment. Plasmids pHVT109 and pHVT115 containing fragment of hup gene of Rhizobium leguminosarum isolated and purified with Qiagen column was obtained. As a probe pHVT115 was labeled with digoxigenin Dutp and dot hybridized with total DNA of 12 Hup\++ A. brasilense strains. The results showed that there was only weak homology between hup genes of most of A. brasilense strains and hup gene contained in pHVT 115. It also revealed that hup gene existed genetic diversity in nitrogen fixing organisms. And heterological genetic probe could not be always suitable for detecting hup genes.\;
【CateGory Index】: S154.32
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Zhao, SONG Xin (School of Life Science, Shandong University, Jinan 250100, China);Research Progress in Anaerobic Fermentative Biohydrogen Production[J];Journal of Agricultural Mechanization Research;2007-10
2 Li Song, Xiaofeng Liu, Yuexiang Yuan, Zhiying Yan, and Yinzhang Liao Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;Progress on Hydrogen-production Microorganisms by Anaerobic Fermentation[J];Chinese Journal of Biotechnology;2008-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Guan Xiuqing; Du Qianyou; Yu Jingzhao(Instifute of Botony, Academia Sinica, Beijing 100093);Isolation and Characteristics of N_2-fixers From Rhizosphere of Plant Leymus chinensis in the Rangeland of Inner Mongolia[J];ACTA AGRESTIA SINICA;1997-02
2 YAO Tuo,ZHANG De-gang,HU Zi-zhi (Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China);Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region Ⅰ Isolation and identification[J];Acta Pratacultural Science;2004-02
3 DENG Yi,LIAO Shu-sheng,CAO Yi,DENG Ri-lie(Foshan University, Foshan 528231, China);Effects of new type ammonia-resistant strains of N_2-fixing bacteria on seeding growth of five vegetables[J];Journal of Foshan University(Natural Science Edition);2005-01
4 HE Li-lan,OU Bing-qing,WEN Hai-xiang,YU Min(Department of Food Science,Foshan University,Nanhai 528231,China);Soaking cucumber seedlings with in aridity stress ammonia-resistant strains of N_2-fixing bacteria for protection[J];Journal of Foshan University(Natural Science Edition);2006-03
5 Qiu Xiaoying, Zhu Honghui, Lu Qiuyan, Qiu Yuansheng (Guangdong Institute of Microbiology, Guangzhou 510070);Study on Stability of Plasmid pMC73A Harbored in host Bacteria[J];HIGH TECHNOLOGY LETTERS;1998-06
6 He Lilan Ou Bingqing Wen Haixiang Chen Huijuan Yu Min (Department of Horticulture, Foshan University, Nanhai 528231, China);Effect of Soaking with Ammonia-resistant Strains of N2-fixing Bacteria on Protective Enzyme of Cucumber Seedling in Chilling Stress[J];Journal of Huazhong Agricultural;2005-03
7 Ping Shuzhen Lin Min You Chongbiao (Institute for Application of Atomic Energy,CAAS10094)Kauser A.Malik (National Institute for Biotechnology and Genetic Engineering,P.O.Box—577,Faisalabad,Pakistan);Salt Tolerance of Diazotrophic Alaligenes Faecalis and Its Interaction with Host Rice under the Salt Stress[J];Journal of Agricultural Biotechnology;1996-01
8 Chen Lei Chen Xiefu Jin Huanxian Gao Huijuan Wang Lingyan(1Dept.of Plant Science, Shanghai Agricultural College, Shanghai 201101;2Agro- Tech Extension Center of Fengxian County, Shanghai 201400);RESEARCH OF MODIFIED RICE GROWTH AND DEVELOPMENT BY IMPROVING THE NITROGEN-FIXING BACTERIA STRAINS[J];;1998-01
9 Zhang Qiulei Lin Min Ping Shuzhen(Biotechnology Research Institute,China Agricultural Science,Beijing 100081);Biological Nitrogen Fixation and Its Application in Sustainable Agriculture[J];Biotechnology Bulletin;2008-02
10 KANG Li-Hua ( Research Institute of Tropical Forestry, Chese Academy of Forestry, Guangzhou 510520);INTERACTION OF ASSOCIATIVE NITROGEN-FIXATION BACTERIA WITH EVCAIYPTUS[J];Microbiology;2002-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LU Wenyu,WEN Jianping,CHEN Yu,LIU Minghui(School of Chemical Engineering & Technology,Tianjin University,Tianjin 300072);Screening of hydrogen-producing aciduric Enterobacter aerogenes strain using He-Ne laser irradiation[J];Chemical Industry and Engineering Progress;2006-07
2 LI Yongfeng, LI Jianzheng, REN Nanqi, LIN Hailong, WANG Xingzu,ZHENG Guoxiang and CUI Yougui(Municipal and Environmental Engineering School, Harbin Institute of Technology,Harbin 150090, Heilongjiang,China);SELECTION OF HIGH EFFICIENCY HYDROGEN—PRODUCING STRAINS OF ANAEROBES BY ULTRA—VIOLET RADIATION[J];Journal fo Chemical Industry and Engineering;2004-S1
3 ZHAO Chun fang;KUANG Sheng lu;XI Qiang;Study of hydrogen production by anaerobic fermentation of glucose disposed by digested sludge[J];Journal of Chemical Industry & Engineering;2001-04
4 Chen Hongzhang,Wang Hui,Zhang Aijun,Li Zuohu(State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100080,China);Biogasification of steam-exploded wheat straw by a two-phased digestion system[J];Transactions of The Chinese Society of Agricultural Engineering;2005-11
5 LI Qiang and WU Yong Qiang * ( Shanghai Institute of Plant physiology, the Chinese Academy of Sciences, Shanghai 200032, China );Mutagenesis of hup T Gene and H 2 uptake Hydrogenase Expression in Photosynthetic Bacterium SDH2[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1999-03
6 LU Wen-yu LIU Ming-hui CHEN Yu WEN Jian-ping (Department of Bioengineering, School of Chemical Engineering & Technology, Tianjin University Tianjin 300072, China);Research Process of Anaerobic Fermentative Hydrogen Production and Its Development Future[J];China Biotechnology;2006-07
7 Li Dongmin Chen Hongzhang Li Zuohu(Key State Lab of Biochemical Engineering Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080);Research and Development of Hydrogen Production by Biological Technology[J];Biotechnology Information;2003-04
8 Cui Yougui,Li Yongfeng,Ren Nanqi et al (Municipal and Environmental Engineering School,Harbin Institute of Technology,Harbin Heilongjiang,150090);The techniques of genetic mutation for bacterium on bio-hydrogen production by beet sugar molasses[J];China Beet & Sugar;2004-03
9 Li Jianzheng,Ren Nanqi,Lin Ming,Wang Yong (School of Municipial and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China);HYDROGEN BIO-PRODUCTION BY ANAEROBIC FERMENTATION OF ORGANIC WASTEWATER IN PILOT-SCALE[J];Acta Energiae Solaris Sinica;2002-02
10 Ren Nanqi 1,Lin Ming 1,Ma Xiping 2,Wang Aijie 1,Li Jianzhen 1 (1. School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; 2. Department of Environmental Science, Liaoning University, Shenyang 110036, China);A STRAIN OF ANAEROBIC BACTERIA SCREENED FOR HIGH EFFICIENT HYDROGEN PRODUCTION AND ITS ACIDURIC CHARACTER[J];Acta Energiae Solaris Sinica;2003-01
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 LI Yuyou1,2,CHU Chunfeng3,AKUTSU Yohei11.Graduate School of Environmental Studies,Tohoku University,Sendai 980-85792. Department of Environmental and Municipal Engineering,Tianjin Institute of Urban Construction,Tianjin 3003843. School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240;Bacterial community and process development for anaerobic hydrogen fermentation[J];Acta Scientiae Circumstantiae;2009-08
2 LI Tao1,SUN Xue-xi1,2,ZHOU Shu-zhen1,JIANG Zhen-xi1,FAN Yao-ting1,REN Bao-zeng1 (1.School of Chemical Engineering,Zhengzhou University,Zhengzhou 450001,China; 2.School of Chemical Engineering and Food,Zhongzhou University,Zhengzhou 450044,China);Study on the scale-up experiment on bio-hydrogen production from corn stalk by anaerobic fermentation[J];Renewable Energy Resources;2009-05
3 Huo Danqun1 Wu Linlin1 Liao Qiang2 Wang Yongzhong2 Hou Changjun1 Tian Xin2(1Bioengineering Department,Chongqing University,Chongqing 400044;2College of Power Engineering,Chongqing Univercity,Chongqing 400044);Selection of Fermentative Hydrogen Producing Bacterium and Its Influencing Factors[J];Biotechnology Bulletin;2009-09
4 Jianchang Li,Wudi Zhang,Fang Yin,Rui Xu,Yubao Chen(Key Laboratory of Advanced Technique and Preparation for Renewable Energy Materials,Ministry of Education,in Yunnan Normal University,Kunming 650092,China);Electrochemically active microorganisms and electrolytically assisted fermentative hydrogen production-A review[J];Acta Microbiologica Sinica;2009-06
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