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《Journal of Microbiology》 2005-01
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Isolation and Characterization of Human Rotavirus HRB-02 Strain

SONG Yan1,WEI Li-li1,CHENG Zhi2(1. Lab of Molec. Biol. of Pathogen.; Coll. of Anim. Med. NE Agric. Univ. Harbin 150040;2. Teach. & Res. Sect. of Microbiol. Coll. of Basic Med.; Harbin Med. Univ. Harbin 150036)  
A human rotavirus was successfully isolated from dejection of a child diarrhea patient in Second College of Clinic Medicine, Harbin Medical University. MA104 cell were used adopting generation passing method, when it came to the forth generation, it appeared obvious cytopathogenic effects (CPE). Typical rotavirus particles were seen under electronic microscope. Its RNA electrophoretic type was 4∶2∶3∶2 accorded with the electrophoretic type of rotavirus group A, therefore, it belongs to rotavirus group A. Initial research study on characteristic of plague and hemagglutination were carried out, and named it as HRB-02. Thus laid the foundation for further study on its molecular biological characteristics and diagnostic antigen and vaccine.
【CateGory Index】: R373
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