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《Journal of Microbiology》 2013-03
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Screening of Cyanide-Tolerance Antagonistic Actinomycetes against Agrobacterium tumefaciens & Its Antimicrobial Action

WANG Shu-fang,JI Jing-lin,XUE Hong-yan,MA Huan-pu,LIU Zhi-min(Coll.of Plant Sci.& Technol.,Beijing Uni.of Agric.,Beijing 102206)  
In order to select a cyanide-resistant and anti peach crown gall biocontrol bacterium.Antagonistic Actinomycetes were isolated by the different concentrations of KCN in culture medium and screened by punching assay.Cyanide-resistant and antagonistic Actinomycetes strain G-19 was screened for the bioactivity using Agrobacterium tumefaciens(At) as a target strain through the plating confrontation culture method.The fermentation broth of G-19 had a high inhibitory effect on At with an inhibition ring at 21.66 mm in diameter.The bacteriostatic action mainly shown in follow aspects: the crude extract reduced the growth logarithmic phase of At,and maintained the mycelium density in a low level.Moreover,the cell wall was curving,wizening,followed by the structure rupture,cytoplasm condensate to the margin,the center etherealized,even formed a cavity;the membrane permeability increased and cause cellular inner substance of At to release;no damage however on DNA of pathogenic bacteria was seen.Therefore,it can be adjudged that the inhibitory effect mechanism of G-19 against At was to destroy the structure of cell wall and to increase the permeability of cell membrane.
【Fund】: 北京市自然科学基金(5112010);; 北京市教委科技计划项目(KM200910020001)
【CateGory Index】: S436.621
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